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Optics Letters

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  • Vol. 36, Iss. 15 — Aug. 1, 2011
  • pp: 2794–2796

Wide-field high-resolution structured illumination solid immersion fluorescence microscopy

Lin Wang, Mark C. Pitter, and Michael G. Somekh  »View Author Affiliations


Optics Letters, Vol. 36, Issue 15, pp. 2794-2796 (2011)
http://dx.doi.org/10.1364/OL.36.002794


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Abstract

The use of aplanatic solid immersion lenses (ASILs) made of high-refractive-index optical materials provides a route to wide-field high-resolution optical microscopy. Structured illumination microscopy (SIM) can double the spatial bandwidth of a microscope to also achieve high-resolution imaging. We investigate the combination of ASILs and SIM in fluorescence microscopy, which we call structured illumination solid immersion fluorescence microscopy (SISIM), to pursue a microscopic system with very large NA and high lateral resolution. We demonstrate that the combination can produce a wide-field high-resolution microscopic system with bandwidth corresponding to an NA of 3. Future developments of the SISIM system to make it achieve even higher resolution are proposed.

© 2011 Optical Society of America

OCIS Codes
(100.2960) Image processing : Image analysis
(110.0110) Imaging systems : Imaging systems
(110.0180) Imaging systems : Microscopy
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(180.2520) Microscopy : Fluorescence microscopy
(350.5730) Other areas of optics : Resolution

ToC Category:
Microscopy

History
Original Manuscript: December 3, 2010
Revised Manuscript: April 23, 2011
Manuscript Accepted: June 6, 2011
Published: July 20, 2011

Virtual Issues
Vol. 6, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Lin Wang, Mark C. Pitter, and Michael G. Somekh, "Wide-field high-resolution structured illumination solid immersion fluorescence microscopy," Opt. Lett. 36, 2794-2796 (2011)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-36-15-2794


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