OSA's Digital Library

Optics Letters

Optics Letters


  • Vol. 36, Iss. 15 — Aug. 1, 2011
  • pp: 2865–2867

In vivo video rate multiphoton microscopy imaging of human skin

Anthony M. D. Lee, Hequn Wang, Yingqiu Yu, Shuo Tang, Jianhua Zhao, Harvey Lui, David I. McLean, and Haishan Zeng  »View Author Affiliations

Optics Letters, Vol. 36, Issue 15, pp. 2865-2867 (2011)

View Full Text Article

Enhanced HTML    Acrobat PDF (779 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We present a multiphoton microscopy instrument specially designed for in vivo dermatological use that is capable of imaging human skin at 27 frames per second with 256 pixels × 256 pixels resolution without the use of exogenous contrast agents. Imaging at fast frame rates is critical to reducing image blurring due to patient motion and to providing practically short clinical measurement times. Second harmonic generation and two-photon fluorescence images and videos acquired at optimized wavelengths are presented showing cellular and tissue structures from the skin surface down to the reticular dermis.

© 2011 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(190.4180) Nonlinear optics : Multiphoton processes
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: May 5, 2011
Revised Manuscript: June 10, 2011
Manuscript Accepted: June 23, 2011
Published: July 25, 2011

Virtual Issues
Vol. 6, Iss. 9 Virtual Journal for Biomedical Optics

Anthony M. D. Lee, Hequn Wang, Yingqiu Yu, Shuo Tang, Jianhua Zhao, Harvey Lui, David I. McLean, and Haishan Zeng, "In vivo video rate multiphoton microscopy imaging of human skin," Opt. Lett. 36, 2865-2867 (2011)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. B. R. Masters, P. T. So, and E. Gratton, Biophys. J. 72, 2405 (1997). [CrossRef] [PubMed]
  2. K. Konig, J. Biophotonics 1, 13 (2008). [CrossRef]
  3. T.-H. Tsai, S.-H. Jee, C.-Y. Dong, and S.-J. Lin, J. Dermatol. Sci. 56, 1 (2009). [CrossRef] [PubMed]
  4. F. Helmchen and W. Denk, Nat. Methods 2, 932 (2005). [CrossRef] [PubMed]
  5. S.-Y. Chen, H.-Y. Wu, and C.-K. Sun, J. Biomed. Opt. 14, 060505 (2009). [CrossRef]
  6. T. Yasui, Y. Takahashi, M. Ito, S. Fukushima, and T. Araki, Appl. Opt. 48, D88 (2009). [CrossRef] [PubMed]
  7. A. N. Bader, A.-M. Pena, C. J. van Voskuilen, J. A. Palero, F. Leroy, A. Colonna, and H. C. Gerritsen, Biomed. Opt. Express 2, 365 (2011). [CrossRef] [PubMed]
  8. E. Dimitrow, M. Ziemer, M. J. Koehler, J. Norgauer, K. Konig, P. Elsner, and M. Kaatz, J. Invest. Dermatol. 129, 1752 (2009). [CrossRef] [PubMed]
  9. M. J. Koehler, S. Hahn, A. Preller, P. Elsner, M. Ziemer, A. Bauer, K. Konig, R. Buckle, J. W. Fluhr, and M. Kaatz, Exp. Dermatol. 17, 519 (2008). [CrossRef] [PubMed]
  10. K. Konig, A. Ehlers, F. Stracke, and I. Riemann, Skin Pharmacol. Physiol. 19, 78 (2006). [CrossRef] [PubMed]
  11. K. Konig, M. Speicher, M. J. Kohler, R. Scharenberg, and M. Kaatz, J. Biophotonics 3, 759 (2010). [CrossRef] [PubMed]
  12. G. Y. Fan, H. Fujisaki, A. Miyawaki, R.-K. Tsay, R. Y. Tsien, and M. H. Ellisman, Biophys. J. 76, 2412 (1999). [CrossRef] [PubMed]
  13. B. Yu, K. Hean Kim, P. T. C. So, D. Blankschtein, and R. Langer, J. Invest. Dermatol. 118, 1085 (2002). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2 Fig. 3
Fig. 4

Supplementary Material

» Media 1: AVI (3904 KB)     
» Media 2: AVI (4038 KB)     
» Media 3: AVI (3910 KB)     
» Media 4: AVI (2285 KB)     

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited