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Optics Letters

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  • Vol. 36, Iss. 15 — Aug. 1, 2011
  • pp: 2865–2867

In vivo video rate multiphoton microscopy imaging of human skin

Anthony M. D. Lee, Hequn Wang, Yingqiu Yu, Shuo Tang, Jianhua Zhao, Harvey Lui, David I. McLean, and Haishan Zeng  »View Author Affiliations


Optics Letters, Vol. 36, Issue 15, pp. 2865-2867 (2011)
http://dx.doi.org/10.1364/OL.36.002865


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Abstract

We present a multiphoton microscopy instrument specially designed for in vivo dermatological use that is capable of imaging human skin at 27 frames per second with 256 pixels × 256 pixels resolution without the use of exogenous contrast agents. Imaging at fast frame rates is critical to reducing image blurring due to patient motion and to providing practically short clinical measurement times. Second harmonic generation and two-photon fluorescence images and videos acquired at optimized wavelengths are presented showing cellular and tissue structures from the skin surface down to the reticular dermis.

© 2011 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(190.4180) Nonlinear optics : Multiphoton processes
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: May 5, 2011
Revised Manuscript: June 10, 2011
Manuscript Accepted: June 23, 2011
Published: July 25, 2011

Virtual Issues
Vol. 6, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Anthony M. D. Lee, Hequn Wang, Yingqiu Yu, Shuo Tang, Jianhua Zhao, Harvey Lui, David I. McLean, and Haishan Zeng, "In vivo video rate multiphoton microscopy imaging of human skin," Opt. Lett. 36, 2865-2867 (2011)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-36-15-2865


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