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  • Vol. 36, Iss. 16 — Aug. 15, 2011
  • pp: 3051–3053

Full-field supercritical angle fluorescence microscopy for live cell imaging

Thomas Barroca, Karla Balaa, Julie Delahaye, Sandrine Lévêque-Fort, and Emmanuel Fort  »View Author Affiliations


Optics Letters, Vol. 36, Issue 16, pp. 3051-3053 (2011)
http://dx.doi.org/10.1364/OL.36.003051


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Abstract

We introduce a full-field fluorescence imaging technique with axial confinement of about 100 nm at the sample/substrate interface. Contrary to standard surface imaging techniques, this confinement is obtained through emission filtering. This technique is based on supercritical emission selectivity. It can be implemented on any epifluorescence microscope with a commercial high numerical aperture objective and offers a real-time surface imaging capability. This technique is of particular interest for live cell membrane and adhesion studies. Using human embryonic kidney cells, we show that one can observe simultaneously the surface and in-depth cell phenomena.

© 2011 Optical Society of America

OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(220.0220) Optical design and fabrication : Optical design and fabrication
(240.0240) Optics at surfaces : Optics at surfaces
(260.6970) Physical optics : Total internal reflection

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: May 12, 2011
Revised Manuscript: June 17, 2011
Manuscript Accepted: July 11, 2011
Published: August 5, 2011

Virtual Issues
Vol. 6, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Thomas Barroca, Karla Balaa, Julie Delahaye, Sandrine Lévêque-Fort, and Emmanuel Fort, "Full-field supercritical angle fluorescence microscopy for live cell imaging," Opt. Lett. 36, 3051-3053 (2011)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-36-16-3051


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