Expand this Topic clickable element to expand a topic
Skip to content
Optica Publishing Group

Single-molecule detection using continuous wave excitation of two-photon fluorescence

Not Accessible

Your library or personal account may give you access

Abstract

Two-photon fluorescence (TPF) is one of the most important discoveries for biological imaging. Although a cw laser is known to excite TPF, its application in TPF imaging has been very limited due to the perceived low efficiency of excitation. Here we directly excited fluorophores with an IR cw laser used for optical trapping and achieved single-molecule fluorescence sensitivity: discrete stepwise photobleaching of enhanced green fluorescent proteins was observed. The single-molecule fluorescence intensity analysis and on-time distribution strongly indicate that a cw laser can generate TPF detectable at the single-molecule level, and thus opens the door to single-molecule TPF imaging using cw lasers.

© 2011 Optical Society of America

Full Article  |  PDF Article
More Like This
Detection of single fluorescent proteins inside eukaryotic cells using two-photon fluorescence

Ximiao Hou and Wei Cheng
Biomed. Opt. Express 3(2) 340-353 (2012)

Single-molecule detection by two-photon-excited fluorescence

J. Mertz, C. Xu, and W. W. Webb
Opt. Lett. 20(24) 2532-2534 (1995)

Two-photon fluorescence excitation in continuous-wave infrared optical tweezers

Y. Liu, G. J. Sonek, M. W. Berns, K. Konig, and B. J. Tromberg
Opt. Lett. 20(21) 2246-2248 (1995)

Cited By

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Figures (4)

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an Optica member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Login to access Optica Member Subscription

Select as filters


Select Topics Cancel
© Copyright 2024 | Optica Publishing Group. All Rights Reserved