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Optics Letters

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  • Vol. 36, Iss. 17 — Sep. 1, 2011
  • pp: 3317–3319

Deciphering fluorescence signals by quantifying separately the excitation intensity from the number of emitters

Richard Hostein, Heykel Aouani, Hervé Rigneault, and Jérôme Wenger  »View Author Affiliations


Optics Letters, Vol. 36, Issue 17, pp. 3317-3319 (2011)
http://dx.doi.org/10.1364/OL.36.003317


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Abstract

Conventional fluorescence detection is sensitive to an intricate product of the number of fluorescent emitters times the local excitation intensity. Here, we describe a method to locally quantify the excitation intensity and the number of emitters separately, enabling a clear distinction between the phenomena responsible for a given fluorescence signal. Our technique is based on harmonic excitation modulation and higher-order fluorescence demodulation. It is compatible with a wide range of observations, opening new characterization routes for fluorescence detection or imaging on complex systems, such as plasmonic substrates or scattering media.

© 2011 Optical Society of America

OCIS Codes
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(180.2520) Microscopy : Fluorescence microscopy
(240.6490) Optics at surfaces : Spectroscopy, surface
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence

ToC Category:
Microscopy

History
Original Manuscript: June 30, 2011
Revised Manuscript: July 21, 2011
Manuscript Accepted: July 21, 2011
Published: August 18, 2011

Virtual Issues
Vol. 6, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Richard Hostein, Heykel Aouani, Hervé Rigneault, and Jérôme Wenger, "Deciphering fluorescence signals by quantifying separately the excitation intensity from the number of emitters," Opt. Lett. 36, 3317-3319 (2011)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-36-17-3317

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