OSA's Digital Library

Optics Letters

Optics Letters


  • Vol. 36, Iss. 17 — Sep. 1, 2011
  • pp: 3317–3319

Deciphering fluorescence signals by quantifying separately the excitation intensity from the number of emitters

Richard Hostein, Heykel Aouani, Hervé Rigneault, and Jérôme Wenger  »View Author Affiliations

Optics Letters, Vol. 36, Issue 17, pp. 3317-3319 (2011)

View Full Text Article

Enhanced HTML    Acrobat PDF (339 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



Conventional fluorescence detection is sensitive to an intricate product of the number of fluorescent emitters times the local excitation intensity. Here, we describe a method to locally quantify the excitation intensity and the number of emitters separately, enabling a clear distinction between the phenomena responsible for a given fluorescence signal. Our technique is based on harmonic excitation modulation and higher-order fluorescence demodulation. It is compatible with a wide range of observations, opening new characterization routes for fluorescence detection or imaging on complex systems, such as plasmonic substrates or scattering media.

© 2011 Optical Society of America

OCIS Codes
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(180.2520) Microscopy : Fluorescence microscopy
(240.6490) Optics at surfaces : Spectroscopy, surface
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence

ToC Category:

Original Manuscript: June 30, 2011
Revised Manuscript: July 21, 2011
Manuscript Accepted: July 21, 2011
Published: August 18, 2011

Virtual Issues
Vol. 6, Iss. 10 Virtual Journal for Biomedical Optics

Richard Hostein, Heykel Aouani, Hervé Rigneault, and Jérôme Wenger, "Deciphering fluorescence signals by quantifying separately the excitation intensity from the number of emitters," Opt. Lett. 36, 3317-3319 (2011)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. J. R. Lakowicz, Principles of Fluorescence Spectroscopy, 3rd ed. (Springer, 2006). [CrossRef]
  2. K. Aslan, I. Gryczynski, J. Malicka, E. Matveeva, J. R. Lakowicz, and C. D. Geddes, Curr. Opin. Biotechnol. 16, 55 (2005). [CrossRef] [PubMed]
  3. E. Fort and S. Grésillon, J. Phys. D 41, 013001 (2008). [CrossRef]
  4. E. Le Moal, E. Fort, S. Lévêque-Fort, F. P. Cordelières, M.-P. Fontaine-Aupart, and C. Ricolleau, Biophys. J. 92, 2150 (2007). [CrossRef]
  5. X. Cui, K. Tawa, H. Hori, and J. Nishii, Adv. Funct. Mater. 20, 546 (2010). [CrossRef]
  6. I. M. Vellekoop, A. Lagendijk, and A. P. Mosk, Nat. Photon. 4, 320 (2010). [CrossRef]
  7. F. Helmchen and W. Denk, Nat. Methods 2, 932 (2005). [CrossRef] [PubMed]
  8. K. Fujita, M. Kobayashi, S. Kawano, M. Yamanaka, and S. Kawata, Phys. Rev. Lett. 99, 228105 (2007). [CrossRef]
  9. S. Kawano, N. I. Smith, M. Yamanaka, S. Kawata, and K. Fujita, Appl. Phys. Express 4, 042401 (2011). [CrossRef]
  10. M. Yamanaka, S. Kawano, K. Fujita, N. I. Smith, and S. Kawata, J. Biomed. Opt. 13, 050507 (2008). [CrossRef] [PubMed]
  11. J. Wenger, D. Gérard, J. Dintinger, O. Mahboub, N. Bonod, E. Popov, T. W. Ebbesen, and H. Rigneault, Opt. Express 16, 3008 (2008). [CrossRef] [PubMed]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1 Fig. 2

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited