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Optics Letters

Optics Letters


  • Vol. 36, Iss. 17 — Sep. 1, 2011
  • pp: 3323–3325

Spectral contrast imaging microscopy

Sergey A. Alexandrov, Shikhar Uttam, Rajan K. Bista, and Yang Liu  »View Author Affiliations

Optics Letters, Vol. 36, Issue 17, pp. 3323-3325 (2011)

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We introduce a new technique, spectral contrast imaging microscopy (SCIM), for super-resolution microscopic imaging. Based on a novel contrast mechanism that encodes each local spatial frequency with a corresponding optical wavelength, SCIM provides a real-time high-resolution spectral contrast microscopic image with superior contrast. We show that two microscopic objects, separated by a distance smaller than the diffraction limit of the optical system, can be spatially resolved in the SCIM image as different colors. Results with numerical simulation and experiments using a high-resolution United States Air Force target are presented. The ability of SCIM for imaging biological cells is also demonstrated.

© 2011 Optical Society of America

OCIS Codes
(070.6110) Fourier optics and signal processing : Spatial filtering
(100.6640) Image processing : Superresolution
(110.0180) Imaging systems : Microscopy
(110.4850) Imaging systems : Optical transfer functions

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: June 1, 2011
Revised Manuscript: July 18, 2011
Manuscript Accepted: July 28, 2011
Published: August 19, 2011

Virtual Issues
Vol. 6, Iss. 10 Virtual Journal for Biomedical Optics

Sergey A. Alexandrov, Shikhar Uttam, Rajan K. Bista, and Yang Liu, "Spectral contrast imaging microscopy," Opt. Lett. 36, 3323-3325 (2011)

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