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Adaptive optics microscopy with direct wavefront sensing using fluorescent protein guide stars |
Optics Letters, Vol. 36, Issue 17, pp. 3389-3391 (2011)
http://dx.doi.org/10.1364/OL.36.003389
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Abstract
We introduce a direct wavefront sensing method using structures labeled with fluorescent proteins in tissues as guide stars. An adaptive optics confocal microscope using this method is demonstrated for imaging of mouse brain tissue. A dendrite and a cell body of a neuron labeled with yellow fluorescent protein are tested as guide stars without injection of other fluorescent labels. Photobleaching effects are also analyzed. The results shows increased image contrast and
© 2011 Optical Society of America
OCIS Codes
(010.7350) Atmospheric and oceanic optics : Wave-front sensing
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(110.1080) Imaging systems : Active or adaptive optics
ToC Category:
Imaging Systems
History
Original Manuscript: May 17, 2011
Revised Manuscript: July 20, 2011
Manuscript Accepted: August 8, 2011
Published: August 24, 2011
Virtual Issues
Vol. 6, Iss. 10 Virtual Journal for Biomedical Optics
Citation
Xiaodong Tao, Oscar Azucena, Min Fu, Yi Zuo, Diana C. Chen, and Joel Kubby, "Adaptive optics microscopy with direct wavefront sensing using fluorescent protein guide stars," Opt. Lett. 36, 3389-3391 (2011)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-36-17-3389
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