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Optics Letters

Optics Letters


  • Vol. 36, Iss. 17 — Sep. 1, 2011
  • pp: 3389–3391

Adaptive optics microscopy with direct wavefront sensing using fluorescent protein guide stars

Xiaodong Tao, Oscar Azucena, Min Fu, Yi Zuo, Diana C. Chen, and Joel Kubby  »View Author Affiliations

Optics Letters, Vol. 36, Issue 17, pp. 3389-3391 (2011)

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We introduce a direct wavefront sensing method using structures labeled with fluorescent proteins in tissues as guide stars. An adaptive optics confocal microscope using this method is demonstrated for imaging of mouse brain tissue. A dendrite and a cell body of a neuron labeled with yellow fluorescent protein are tested as guide stars without injection of other fluorescent labels. Photobleaching effects are also analyzed. The results shows increased image contrast and 3 × improvement in the signal intensity for fixed mouse tissues at depths of 70 μm .

© 2011 Optical Society of America

OCIS Codes
(010.7350) Atmospheric and oceanic optics : Wave-front sensing
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Imaging Systems

Original Manuscript: May 17, 2011
Revised Manuscript: July 20, 2011
Manuscript Accepted: August 8, 2011
Published: August 24, 2011

Virtual Issues
Vol. 6, Iss. 10 Virtual Journal for Biomedical Optics

Xiaodong Tao, Oscar Azucena, Min Fu, Yi Zuo, Diana C. Chen, and Joel Kubby, "Adaptive optics microscopy with direct wavefront sensing using fluorescent protein guide stars," Opt. Lett. 36, 3389-3391 (2011)

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