OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 36, Iss. 17 — Sep. 1, 2011
  • pp: 3389–3391

Adaptive optics microscopy with direct wavefront sensing using fluorescent protein guide stars

Xiaodong Tao, Oscar Azucena, Min Fu, Yi Zuo, Diana C. Chen, and Joel Kubby  »View Author Affiliations


Optics Letters, Vol. 36, Issue 17, pp. 3389-3391 (2011)
http://dx.doi.org/10.1364/OL.36.003389


View Full Text Article

Enhanced HTML    Acrobat PDF (462 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We introduce a direct wavefront sensing method using structures labeled with fluorescent proteins in tissues as guide stars. An adaptive optics confocal microscope using this method is demonstrated for imaging of mouse brain tissue. A dendrite and a cell body of a neuron labeled with yellow fluorescent protein are tested as guide stars without injection of other fluorescent labels. Photobleaching effects are also analyzed. The results shows increased image contrast and 3 × improvement in the signal intensity for fixed mouse tissues at depths of 70 μm .

© 2011 Optical Society of America

OCIS Codes
(010.7350) Atmospheric and oceanic optics : Wave-front sensing
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.1790) Microscopy : Confocal microscopy
(180.2520) Microscopy : Fluorescence microscopy
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Imaging Systems

History
Original Manuscript: May 17, 2011
Revised Manuscript: July 20, 2011
Manuscript Accepted: August 8, 2011
Published: August 24, 2011

Virtual Issues
Vol. 6, Iss. 10 Virtual Journal for Biomedical Optics

Citation
Xiaodong Tao, Oscar Azucena, Min Fu, Yi Zuo, Diana C. Chen, and Joel Kubby, "Adaptive optics microscopy with direct wavefront sensing using fluorescent protein guide stars," Opt. Lett. 36, 3389-3391 (2011)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-36-17-3389


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. M. J. Booth, Phil. Trans. R. Soc. A 365, 2829 (2007). [CrossRef] [PubMed]
  2. D. Debarre, E. J. Botcherby, T. Watanabe, S. Srinivas, M. J. Booth, and T. Wilson, Opt. Lett. 34, 2495 (2009). [CrossRef] [PubMed]
  3. M. Rueckel, J. A. Mack-Bucher, and W. Denk, Proc. Natl. Acad. Sci. USA 103, 17137 (2006). [CrossRef] [PubMed]
  4. O. Azucena, J. Crest, S. Kotadia, W. Sullivan, X. Tao, M. Reinig, D. Gavel, S. Olivier, and J. Kubby, Opt. Lett. 36, 825 (2011). [CrossRef] [PubMed]
  5. X. Tao, B. Fernandez, O. Azucena, M. Fu, D. Garcia, Y. Zuo, D. Chen, and J. Kubby, Opt. Lett. 36, 1062 (2011). [CrossRef] [PubMed]
  6. O. Azucena, J. Crest, J. Cao, W. Sullivan, P. Kner, D. Gavel, D. Dillon, S. Olivier, and J. Kubby, Opt. Express 18, 17521 (2010). [CrossRef] [PubMed]
  7. D. P. Biss, D. Sumorok, S. A. Burns, R. H. Webb, Y. Zhou, T. G. Bifano, D. Côté, I. Veilleux, P. Zamiri, and C. P. Lin, Opt. Lett. 32, 659 (2007). [CrossRef] [PubMed]
  8. L. Diaz Santana Haro and J. C. Dainty, Opt. Lett. 24, 61(1999). [CrossRef]
  9. J. R. Lakowicz, Principles of Fluorescence Spectroscopy (Springer, 2006). [CrossRef]
  10. J. Beverage, R. Shack, and M. Descour, J. Microsc. 205, 61 (2002). [CrossRef] [PubMed]
  11. S. Thomas, T. Fusco, A. Tokovinin, M. Nicolle, V. Michau, and G. Rousset, Mon. Not. R. Astron. Soc. 371, 323 (2006). [CrossRef]
  12. L. A. Poyneer, D. T. Gavel, and J. M. Brase, J. Opt. Soc. Am. A 19, 2100 (2002). [CrossRef]
  13. O. Azucena, X. Tao, J. Crest, S. Kotadia, W. Sullivan, D. Gavel, M. Reinig, S. Olivier, and J. Kubby, Proc. SPIE 7931, 79310J (2011). [CrossRef]
  14. K. Morzinski, L. C. Johnson, D. T. Gavel, B. Grigsby, D. Dillon, M. Reinig, and B. A. Macintosh, Proc. SPIE 7736, 77361O (2010). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 2 Fig. 3
 
Fig. 4
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited