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Three-dimensional in vivo scanning microscopy with inertia-free focus control |
Optics Letters, Vol. 36, Issue 17, pp. 3503-3505 (2011)
http://dx.doi.org/10.1364/OL.36.003503
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Abstract
The acquisition of high-resolution images in three dimensions is of utmost importance for the morphological and functional investigation of biological tissues. Here, we present a laser scanning two-photon microscope with remote and motionless control of the focus position. The movement of the excitation spot along the propagation direction is achieved by shaping the laser wavefront with a spatial light modulator. Depending on the optical properties of the objective in use, this approach allows z movements in a range of tens to hundreds of micrometers with small changes of the point spread function. We applied this technique for the three-dimensional (3D) imaging of fluorescent cells in the mouse neocortex in vivo. The presented system bypasses the limitations of microscopes based on moving objectives, enabling high-resolution inertia-free 3D imaging.
© 2011 Optical Society of America
OCIS Codes
(090.1995) Holography : Digital holography
(180.4315) Microscopy : Nonlinear microscopy
(070.6120) Fourier optics and signal processing : Spatial light modulators
ToC Category:
Fourier Optics and Signal Processing
History
Original Manuscript: July 22, 2011
Manuscript Accepted: August 8, 2011
Published: September 1, 2011
Virtual Issues
Vol. 6, Iss. 10 Virtual Journal for Biomedical Optics
Citation
Marco Dal Maschio, Angela Michela De Stasi, Fabio Benfenati, and Tommaso Fellin, "Three-dimensional in vivo scanning microscopy with inertia-free focus control," Opt. Lett. 36, 3503-3505 (2011)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-36-17-3503
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