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Optics Letters


  • Vol. 36, Iss. 18 — Sep. 15, 2011
  • pp: 3620–3622

Diagnostic value of nonlinear optical signals from collagen matrix in the detection of epithelial precancer

Wei Zheng, Dong Li, Shuxia Li, Yan Zeng, Yanqi Yang, and Jianan Y. Qu  »View Author Affiliations

Optics Letters, Vol. 36, Issue 18, pp. 3620-3622 (2011)

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During precancer development in epithelium, neoplastic cells remodel the underlying stroma, for example, the basement membrane, capillaries, fibroblasts, and extracellular matrix. The purpose of this study is to investigate the relationship between the nonlinear optical signals from the collagen matrix in stroma and the progression of early epithelial carcinogenesis. Two-photon excited fluorescence (TPEF) and second harmonic generation (SHG) signals were measured from the stroma of hamster oral cheek pouch in vivo. We found that three features, including the intensity ratio of TPEF over SHG, the spatial frequency distribution, and the texture feature of SHG images, provide the quantitative identification of epithelial precancer at different pathologic stages. We demonstrated that the combination of all three features by using a support vector machine algorithm can significantly improve the accuracy in the detection of epithelial precancer.

© 2011 Optical Society of America

OCIS Codes
(170.4580) Medical optics and biotechnology : Optical diagnostics for medicine
(170.4730) Medical optics and biotechnology : Optical pathology
(170.6930) Medical optics and biotechnology : Tissue
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: June 28, 2011
Manuscript Accepted: August 2, 2011
Published: September 13, 2011

Virtual Issues
Vol. 6, Iss. 10 Virtual Journal for Biomedical Optics

Wei Zheng, Dong Li, Shuxia Li, Yan Zeng, Yanqi Yang, and Jianan Y. Qu, "Diagnostic value of nonlinear optical signals from collagen matrix in the detection of epithelial precancer," Opt. Lett. 36, 3620-3622 (2011)

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