Toward whole-body optical imaging of rats using single-photon counting fluorescence tomography

doi:10.1364/OL.36.003723

Toward whole-body optical imaging of rats using single-photon counting fluorescence tomography

Frederic Leblond, Kenneth M. Tichauer, Robert W. Holt, Fadi El-Ghussein, and Brian W. Pogue »View Author Affiliations

Optics Letters, Vol. 36, Issue 19, pp. 3723-3725 (2011)
http://dx.doi.org/10.1364/OL.36.003723

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Abstract

We used single-photon counting (SPC) detection for diffuse fluorescence tomography to image nanomolar (nM) concentrations of reporter dyes through a rat. Detailed phantom data are presented to show that every centimeter increase in tissue thickness leads to 1 order of magnitude decrease in the minimum fluorophore concentration detectable for a given detector sensitivity. Specifically, here, detection of Alexa Fluor 647 dyes is shown to be achievable for concentrations as low as $1 nM$ ( $< 200 fM$ ) through more than $5 cm$ in tissue phantoms, which indicates that this is feasible in larger rodent models. Because it is possible to detect sub-nM fluorescent inclusions with SPC technology in rats, it follows that it is possible to localize subpicomolar fluorophore concentrations in mice, putting the concentration sensitivity limits on the same order as nuclear medicine methods.

OCIS Codes
(170.5280) Medical optics and biotechnology : Photon migration
(300.6280) Spectroscopy : Spectroscopy, fluorescence and luminescence
(110.4234) Imaging systems : Multispectral and hyperspectral imaging
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: June 2, 2011
Revised Manuscript: August 19, 2011
Manuscript Accepted: August 24, 2011
Published: September 19, 2011

Virtual Issues
Vol. 6, Iss. 11 Virtual Journal for Biomedical Optics
September 27, 2011 Spotlight on Optics

Citation
Frederic Leblond, Kenneth M. Tichauer, Robert W. Holt, Fadi El-Ghussein, and Brian W. Pogue, "Toward whole-body optical imaging of rats using single-photon counting fluorescence tomography," Opt. Lett. 36, 3723-3725 (2011)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-36-19-3723

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References

F. Leblond, S. C. Davis, P. A. Valdes, and B. W. Pogue, J. Photochem. Photobiol. B 98, 77 (2010). [CrossRef]
D. Kepshire, N. Mincu, M. Hutchins, J. Gruber, H. Dehghani, J. Hypnarowski, F. Leblond, M. Khayat, and B. W. Pogue, Rev. Sci. Instrum. 80, 043701 (2009). [CrossRef] [PubMed]
V. Ntziachristos and R. Weissleder, Opt. Lett. 26, 893(2001). [CrossRef]
H. Dehghani, M. E. Eames, P. K. Yalavarthy, S. C. Davis, S. Srinivasan, C. M. Carpenter, B. W. Pogue, and K. D. Paulsen, Commun. Num. Meth. Eng. 25, 711 (2009). [CrossRef]
D. L. Kepshire, H. Dehghani, F. Leblond, and B. W. Pogue, Opt. Express 17, 23272 (2009). [CrossRef]

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