OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 36, Iss. 2 — Jan. 15, 2011
  • pp: 202–204

Corkscrew point spread function for far-field three-dimensional nanoscale localization of pointlike objects

Matthew D. Lew, Steven F. Lee, Majid Badieirostami, and W. E. Moerner  »View Author Affiliations


Optics Letters, Vol. 36, Issue 2, pp. 202-204 (2011)
http://dx.doi.org/10.1364/OL.36.000202


View Full Text Article

Enhanced HTML    Acrobat PDF (359 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

We describe the corkscrew point spread function (PSF), which can localize objects in three dimensions throughout a 3.2 μm depth of field with nanometer precision. The corkscrew PSF rotates as a function of the axial (z) position of an emitter. Fisher information calculations show that the corkscrew PSF can achieve nanometer localization precision with limited numbers of photons. We demonstrate three-dimensional super-resolution microscopy with the corkscrew PSF by imaging beads on the surface of a triangular polydimethylsiloxane (PDMS) grating. With 99,000 photons detected, the corkscrew PSF achieves a localization precision of 2.7 nm in x, 2.1 nm in y, and 5.7 nm in z.

© 2011 Optical Society of America

OCIS Codes
(110.4850) Imaging systems : Optical transfer functions
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(110.1758) Imaging systems : Computational imaging

ToC Category:
Microscopy

History
Original Manuscript: September 10, 2010
Revised Manuscript: November 18, 2010
Manuscript Accepted: December 10, 2010
Published: January 11, 2011

Virtual Issues
Vol. 6, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Matthew D. Lew, Steven F. Lee, Majid Badieirostami, and W. E. Moerner, "Corkscrew point spread function for far-field three-dimensional nanoscale localization of pointlike objects," Opt. Lett. 36, 202-204 (2011)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-36-2-202

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited