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Optics Letters

Optics Letters


  • Vol. 36, Iss. 2 — Jan. 15, 2011
  • pp: 202–204

Corkscrew point spread function for far-field three-dimensional nanoscale localization of pointlike objects

Matthew D. Lew, Steven F. Lee, Majid Badieirostami, and W. E. Moerner  »View Author Affiliations

Optics Letters, Vol. 36, Issue 2, pp. 202-204 (2011)

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We describe the corkscrew point spread function (PSF), which can localize objects in three dimensions throughout a 3.2 μm depth of field with nanometer precision. The corkscrew PSF rotates as a function of the axial (z) position of an emitter. Fisher information calculations show that the corkscrew PSF can achieve nanometer localization precision with limited numbers of photons. We demonstrate three-dimensional super-resolution microscopy with the corkscrew PSF by imaging beads on the surface of a triangular polydimethylsiloxane (PDMS) grating. With 99,000 photons detected, the corkscrew PSF achieves a localization precision of 2.7 nm in x, 2.1 nm in y, and 5.7 nm in z.

© 2011 Optical Society of America

OCIS Codes
(110.4850) Imaging systems : Optical transfer functions
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(110.1758) Imaging systems : Computational imaging

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Original Manuscript: September 10, 2010
Revised Manuscript: November 18, 2010
Manuscript Accepted: December 10, 2010
Published: January 11, 2011

Virtual Issues
Vol. 6, Iss. 2 Virtual Journal for Biomedical Optics

Matthew D. Lew, Steven F. Lee, Majid Badieirostami, and W. E. Moerner, "Corkscrew point spread function for far-field three-dimensional nanoscale localization of pointlike objects," Opt. Lett. 36, 202-204 (2011)

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