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Optics Letters

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  • Vol. 36, Iss. 2 — Jan. 15, 2011
  • pp: 289–291

Optimal acquisition scheme for subwavelength localization microscopy of bleachable fluorophores

Edward Shore and Alex Small  »View Author Affiliations


Optics Letters, Vol. 36, Issue 2, pp. 289-291 (2011)
http://dx.doi.org/10.1364/OL.36.000289


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Abstract

We consider the problem of optimizing superresolution microscopy with photoswitchable molecules that irreversibly photobleach. Using variational methods, we show that the number of single-molecule images is maximized in a simple scheme with a constant number of activated fluorophores per cycle. For high-speed acquisition, deviations from the optimal scheme do reduce the information collected but fortuitously also reduce certain types of errors, making the scheme very robust. Finally, we calculate the amount of information lost due to bleaching and noise.

© 2011 Optical Society of America

OCIS Codes
(100.6640) Image processing : Superresolution
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Microscopy

History
Original Manuscript: October 13, 2010
Revised Manuscript: December 2, 2010
Manuscript Accepted: December 14, 2010
Published: January 13, 2011

Virtual Issues
Vol. 6, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Edward Shore and Alex Small, "Optimal acquisition scheme for subwavelength localization microscopy of bleachable fluorophores," Opt. Lett. 36, 289-291 (2011)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-36-2-289

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