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Optics Letters

Optics Letters


  • Vol. 36, Iss. 20 — Oct. 15, 2011
  • pp: 3987–3989

Microscopy refocusing and dark-field imaging by using a simple LED array

Guoan Zheng, Christopher Kolner, and Changhuei Yang  »View Author Affiliations

Optics Letters, Vol. 36, Issue 20, pp. 3987-3989 (2011)

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The condenser is one of the main components in most transmitted light compound microscopes. In this Letter, we show that such a condenser can be replaced by a programmable LED array to achieve greater imaging flexibility and functionality. Without mechanically scanning the sample or changing the microscope setup, the proposed approach can be used for dark-field imaging, bright-field imaging, microscopy sectioning, and digital refocusing. Images of a starfish embryo were acquired by using such an approach for demonstration.

© 2011 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(110.2945) Imaging systems : Illumination design
(110.3010) Imaging systems : Image reconstruction techniques

ToC Category:
Imaging Systems

Original Manuscript: July 14, 2011
Revised Manuscript: September 9, 2011
Manuscript Accepted: September 12, 2011
Published: October 5, 2011

Virtual Issues
Vol. 6, Iss. 11 Virtual Journal for Biomedical Optics

Guoan Zheng, Christopher Kolner, and Changhuei Yang, "Microscopy refocusing and dark-field imaging by using a simple LED array," Opt. Lett. 36, 3987-3989 (2011)

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