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  • Vol. 36, Iss. 20 — Oct. 15, 2011
  • pp: 3987–3989

Microscopy refocusing and dark-field imaging by using a simple LED array

Guoan Zheng, Christopher Kolner, and Changhuei Yang  »View Author Affiliations


Optics Letters, Vol. 36, Issue 20, pp. 3987-3989 (2011)
http://dx.doi.org/10.1364/OL.36.003987


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Abstract

The condenser is one of the main components in most transmitted light compound microscopes. In this Letter, we show that such a condenser can be replaced by a programmable LED array to achieve greater imaging flexibility and functionality. Without mechanically scanning the sample or changing the microscope setup, the proposed approach can be used for dark-field imaging, bright-field imaging, microscopy sectioning, and digital refocusing. Images of a starfish embryo were acquired by using such an approach for demonstration.

© 2011 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(110.2945) Imaging systems : Illumination design
(110.3010) Imaging systems : Image reconstruction techniques

ToC Category:
Imaging Systems

History
Original Manuscript: July 14, 2011
Revised Manuscript: September 9, 2011
Manuscript Accepted: September 12, 2011
Published: October 5, 2011

Virtual Issues
Vol. 6, Iss. 11 Virtual Journal for Biomedical Optics

Citation
Guoan Zheng, Christopher Kolner, and Changhuei Yang, "Microscopy refocusing and dark-field imaging by using a simple LED array," Opt. Lett. 36, 3987-3989 (2011)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-36-20-3987


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References

  1. J. James, Light Microscopic Techniques in Biology and Medicine (Springer, 1976). [CrossRef]
  2. M. G. L. Gustafsson, Proc. Natl. Acad. Sci. USA 102, 13081 (2005). [CrossRef] [PubMed]
  3. V. Poher, H. Zhang, G. Kennedy, C. Griffin, S. Oddos, E. Gu, D. Elson, M. Girkin, P. French, and M. Dawson, Opt. Express 15, 11196 (2007). [CrossRef] [PubMed]
  4. J. Huisken, J. Swoger, F. Del Bene, J. Wittbrodt, and E. H. K. Stelzer, Science 305, 1007 (2004). [CrossRef] [PubMed]
  5. J. Wu, G. Zheng, Z. Li, and C. Yang, Opt. Lett. 36, 2179(2011). [CrossRef] [PubMed]
  6. F. O. Fahrbach, P. Simon, and A. Rohrbach, Nat. Photon. 4, 780 (2010). [CrossRef]
  7. V. Bormuth, J. Howard, and E. Schaffer, J. Microsc. 226, 1 (2007). [CrossRef] [PubMed]
  8. V. Murthy, T. F. Sato, D. F. Albeanu, E. Soucy, and M. Meister, PLoS ONE 3 (2008). [PubMed]
  9. G. C. Cox, Optical Imaging Techniques in Cell Biology (CRC/Taylor & Francis, Boca Raton, 2007).
  10. G. Zheng, X. Cui, and C. Yang, in Proceedings of the National Academy of Sciences of India, Section A (Physical Sciences) (2010), Vol. 107, p. 9043.
  11. P. J. Shaw, D. A. Agard, Y. Hiraoka, and J. W. Sedat, Biophys. J. 55, 101 (1989). [CrossRef] [PubMed]
  12. G. Zheng, S. A. Lee, S. Yang, and C. Yang, Lab Chip 10, 3125 (2010). [CrossRef] [PubMed]
  13. A. Barty, K. Nugent, D. Paganin, and A. Roberts, Opt. Lett. 23, 817 (1998). [CrossRef]

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