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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 36, Iss. 21 — Nov. 1, 2011
  • pp: 4206–4208

Pupil-segmentation-based adaptive optical microscopy with full-pupil illumination

Daniel E. Milkie, Eric Betzig, and Na Ji  »View Author Affiliations

Optics Letters, Vol. 36, Issue 21, pp. 4206-4208 (2011)

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Optical aberrations deteriorate the performance of microscopes. Adaptive optics can be used to improve imaging performance via wavefront shaping. Here, we demonstrate a pupil-segmentation based adaptive optical approach with full-pupil illumination. When implemented in a two-photon fluorescence microscope, it recovers diffraction-limited performance and improves imaging signal and resolution.

© 2011 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Imaging Systems

Original Manuscript: July 15, 2011
Revised Manuscript: September 20, 2011
Manuscript Accepted: September 21, 2011
Published: October 25, 2011

Daniel E. Milkie, Eric Betzig, and Na Ji, "Pupil-segmentation-based adaptive optical microscopy with full-pupil illumination," Opt. Lett. 36, 4206-4208 (2011)

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