Optical aberrations deteriorate the performance of microscopes. Adaptive optics can be used to improve imaging performance via wavefront shaping. Here, we demonstrate a pupil-segmentation based adaptive optical approach with full-pupil illumination. When implemented in a two-photon fluorescence microscope, it recovers diffraction-limited performance and improves imaging signal and resolution.
© 2011 Optical Society of America
Original Manuscript: July 15, 2011
Revised Manuscript: September 20, 2011
Manuscript Accepted: September 21, 2011
Published: October 25, 2011
Daniel E. Milkie, Eric Betzig, and Na Ji, "Pupil-segmentation-based adaptive optical microscopy with full-pupil illumination," Opt. Lett. 36, 4206-4208 (2011)