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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 36, Iss. 21 — Nov. 1, 2011
  • pp: 4206–4208

Pupil-segmentation-based adaptive optical microscopy with full-pupil illumination

Daniel E. Milkie, Eric Betzig, and Na Ji  »View Author Affiliations


Optics Letters, Vol. 36, Issue 21, pp. 4206-4208 (2011)
http://dx.doi.org/10.1364/OL.36.004206


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Abstract

Optical aberrations deteriorate the performance of microscopes. Adaptive optics can be used to improve imaging performance via wavefront shaping. Here, we demonstrate a pupil-segmentation based adaptive optical approach with full-pupil illumination. When implemented in a two-photon fluorescence microscope, it recovers diffraction-limited performance and improves imaging signal and resolution.

© 2011 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(110.1080) Imaging systems : Active or adaptive optics

ToC Category:
Imaging Systems

History
Original Manuscript: July 15, 2011
Revised Manuscript: September 20, 2011
Manuscript Accepted: September 21, 2011
Published: October 25, 2011

Citation
Daniel E. Milkie, Eric Betzig, and Na Ji, "Pupil-segmentation-based adaptive optical microscopy with full-pupil illumination," Opt. Lett. 36, 4206-4208 (2011)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-36-21-4206


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References

  1. M. J. Booth, Phil. Trans. R. Soc. A 365, 2829 (2007). [CrossRef] [PubMed]
  2. R. K. Tyson, Principles of Adaptive Optics (Academic, 1991).
  3. J. W. Hardy, Adaptive Optics for Astronomical Telescopes (Oxford University Press, 1998).
  4. N. Ji, D. E. Milkie, and E. Betzig, Nat. Methods 7, 141(2010). [CrossRef]
  5. N. Ji, T. R. Sato, and E. Betzig, “Characterization and adaptive optical correction of aberrations during in vivo imaging in the mouse cortex,” Proc. Acad. Natl. Sci. USA , submitted for publication.
  6. S. I. Panagopoulou and D. R. Neal, J. Refract. Surg. 21, S563 (2005). [PubMed]
  7. M. A. Helmbrecht, M. He, C. J. Kempf, and M. Besse, Proc. SPIE 7931, 793108 (2011). [CrossRef]

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