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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 36, Iss. 21 — Nov. 1, 2011
  • pp: 4242–4244

Resolution enhancement of random adsorbed single-molecule localization based on surface plasmon resonance illumination

Xiaomin Zhai, Yunfei Sun, and Dongmin Wu  »View Author Affiliations

Optics Letters, Vol. 36, Issue 21, pp. 4242-4244 (2011)

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Single-molecule localization (SML) is a powerful tool to overcome the diffraction limit in optical imaging, because the fluorescence emitted by single molecules can be observed with nanometer accuracy when the optical background and associated noise are made sufficiently small. Random adsorbed SML has been successfully demonstrated for superresolution imaging on metal surfaces. To optimize the random adsorbed SML, we developed a new illumination method based on surface plasmon resonance (SPR). The enhancement of the fluorescence signal and the reduction of background noise were achieved simultaneously. A high localization resolution of 15 nm was demonstrated with this new SPR illumination system.

© 2011 Optical Society of America

OCIS Codes
(100.6640) Image processing : Superresolution
(170.2945) Medical optics and biotechnology : Illumination design

ToC Category:
Image Processing

Original Manuscript: July 5, 2011
Revised Manuscript: August 20, 2011
Manuscript Accepted: September 29, 2011
Published: October 27, 2011

Virtual Issues
Vol. 7, Iss. 1 Virtual Journal for Biomedical Optics

Xiaomin Zhai, Yunfei Sun, and Dongmin Wu, "Resolution enhancement of random adsorbed single-molecule localization based on surface plasmon resonance illumination," Opt. Lett. 36, 4242-4244 (2011)

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