OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 36, Iss. 21 — Nov. 1, 2011
  • pp: 4305–4307

Phase-shifting Zernike phase contrast microscopy for quantitative phase measurement

Peng Gao, Baoli Yao, Irina Harder, Norbert Lindlein, and Francisco Jose Torcal-Milla  »View Author Affiliations


Optics Letters, Vol. 36, Issue 21, pp. 4305-4307 (2011)
http://dx.doi.org/10.1364/OL.36.004305


View Full Text Article

Enhanced HTML    Acrobat PDF (410 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Zernike phase contrast microscopy is extended and combined with a phase-shifting mechanism to perform quantitative phase measurements of microscopic objects. Dozens of discrete point light sources on a ring are constructed for illumination. For each point light source, three different levels of point-like phase steps are designed, which are alternatively located along a ring on a silica plate to perform phase retardation on the undiffracted (dc) component of the object waves. These three levels of the phase steps are respectively selected by rotating the silica plate. Thus, quantitative evaluation of phase specimens can be performed via phase-shifting mechanism. The proposed method has low “halo” and “shade-off” effects, low coherent noise level, and high lateral resolution due to the improved illumination scheme.

© 2011 Optical Society of America

OCIS Codes
(070.6110) Fourier optics and signal processing : Spatial filtering
(100.5090) Image processing : Phase-only filters
(170.0180) Medical optics and biotechnology : Microscopy
(180.0180) Microscopy : Microscopy

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: July 14, 2011
Revised Manuscript: September 18, 2011
Manuscript Accepted: September 20, 2011
Published: November 1, 2011

Virtual Issues
Vol. 7, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Peng Gao, Baoli Yao, Irina Harder, Norbert Lindlein, and Francisco Jose Torcal-Milla, "Phase-shifting Zernike phase contrast microscopy for quantitative phase measurement," Opt. Lett. 36, 4305-4307 (2011)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-36-21-4305


Sort:  Author  |  Year  |  Journal  |  Reset  

References

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1 Fig. 2 Fig. 3
 
Fig. 4 Fig. 5
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited