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Optics Letters

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  • Editor: Alan E. Willner
  • Vol. 36, Iss. 23 — Dec. 1, 2011
  • pp: 4704–4706

Laplace field microscopy for label-free imaging of dynamic biological structures

Taewoo Kim and Gabriel Popescu  »View Author Affiliations


Optics Letters, Vol. 36, Issue 23, pp. 4704-4706 (2011)
http://dx.doi.org/10.1364/OL.36.004704


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Abstract

We present Laplace field microscopy as a method for generating intrinsic contrast of transparent specimens. This technique uses a spatial light modulator to perform the Laplacian of the field in the Fourier plane of a microscope image. The resulting image incorporates phase information and thus renders high contrast images from phase objects. We demonstrate the potential of the method by imaging index-matched beads, unlabeled tissue slices, and dynamic live cells.

© 2011 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.1530) Medical optics and biotechnology : Cell analysis
(170.1650) Medical optics and biotechnology : Coherence imaging
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: September 1, 2011
Revised Manuscript: November 1, 2011
Manuscript Accepted: November 1, 2011
Published: December 1, 2011

Virtual Issues
Vol. 7, Iss. 2 Virtual Journal for Biomedical Optics

Citation
Taewoo Kim and Gabriel Popescu, "Laplace field microscopy for label-free imaging of dynamic biological structures," Opt. Lett. 36, 4704-4706 (2011)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-36-23-4704


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