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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 36, Iss. 4 — Feb. 15, 2011
  • pp: 430–432

Spectral-domain differential interference contrast microscopy

Yizheng Zhu, Natan T. Shaked, Lisa L. Satterwhite, and Adam Wax  »View Author Affiliations

Optics Letters, Vol. 36, Issue 4, pp. 430-432 (2011)

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We present a fiber-optic low-coherence imaging technique, termed spectral-domain differential interference contrast microscopy (SD-DIC), for quantitative DIC imaging of both reflective surfaces and transparent biological specimens. SD-DIC combines the common-path nature of a Nomarski DIC interferometer with the high sensitivity of spectral-domain low-coherence interferometry to obtain high-resolution, quantitative measurements of optical pathlength gradients from a single point on the sample. Full-field imaging can be achieved by scanning the sample. A reflected-light SD-DIC system was demonstrated using a USAF resolution target as the phase object. Live cardiomyocytes were also imaged, achieving a resolution of 36 pm for pathlength gradient measurements. The dynamics of cardiomyocyte contraction were recorded with high sensitivity at selected sites on the cells.

© 2011 Optical Society of America

OCIS Codes
(110.0180) Imaging systems : Microscopy
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(170.5810) Medical optics and biotechnology : Scanning microscopy
(180.3170) Microscopy : Interference microscopy

ToC Category:
Imaging Systems

Original Manuscript: October 6, 2010
Revised Manuscript: December 7, 2010
Manuscript Accepted: December 21, 2010
Published: January 31, 2011

Virtual Issues
Vol. 6, Iss. 3 Virtual Journal for Biomedical Optics

Yizheng Zhu, Natan T. Shaked, Lisa L. Satterwhite, and Adam Wax, "Spectral-domain differential interference contrast microscopy," Opt. Lett. 36, 430-432 (2011)

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