OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 36, Iss. 5 — Mar. 1, 2011
  • pp: 663–665

Agitation-free multiphoton microscopy of oblique planes

Christopher W. Smith, Edward J. Botcherby, Martin J. Booth, Rimas Juškaitis, and Tony Wilson  »View Author Affiliations


Optics Letters, Vol. 36, Issue 5, pp. 663-665 (2011)
http://dx.doi.org/10.1364/OL.36.000663


View Full Text Article

Enhanced HTML    Acrobat PDF (352 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

The scanning two-photon fluorescence microscope produces optically sectioned images from the focal plane. It is sometimes desirable to acquire images from other planes of the specimen that are inclined with respect to the focal plane. In this Letter, we discuss the issues concerned with acquiring such images together with the effects of the inclination angle on image resolution and sectioning strength. To obtain images from oblique planes at high speed, a two-photon system was built wherein a novel optical system is used to provide aberration-free scanning.

© 2011 Optical Society of America

OCIS Codes
(180.5810) Microscopy : Scanning microscopy
(180.6900) Microscopy : Three-dimensional microscopy

ToC Category:
Microscopy

History
Original Manuscript: September 24, 2010
Manuscript Accepted: October 28, 2010
Published: February 23, 2011

Virtual Issues
Vol. 6, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Christopher W. Smith, Edward J. Botcherby, Martin J. Booth, Rimas Juškaitis, and Tony Wilson, "Agitation-free multiphoton microscopy of oblique planes," Opt. Lett. 36, 663-665 (2011)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-36-5-663

You do not have subscription access to this journal. Citation lists with outbound citation links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Cited by links are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Figure files are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

You do not have subscription access to this journal. Article level metrics are available to subscribers only. You may subscribe either as an OSA member, or as an authorized user of your institution.

Contact your librarian or system administrator
or
Log in to access OSA Member Subscription

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited