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Single-wavelength functional photoacoustic microscopy in biological tissue |
Optics Letters, Vol. 36, Issue 5, pp. 769-771 (2011)
http://dx.doi.org/10.1364/OL.36.000769
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Abstract
Recently, we developed a reflection-mode relaxation photoacoustic microscope, based on saturation intensity, to measure picosecond relaxation times using a nanosecond laser. Here, using the different relaxation times of oxygenated and deoxygenated hemoglobin molecules, both possessing extremely low fluorescence quantum yields, the oxygen saturation was quantified in vivo with single-wavelength photoacoustic microscopy. All previous functional photoacoustic microscopy measurements required imaging with multiple-laser-wavelength measurements to quantify oxygen saturation. Eliminating the need for multiwavelength measurements removes the influence of spectral properties on oxygenation calculations and improves the portability and cost-effectiveness of functional or molecular photoacoustic microscopy.
© 2011 Optical Society of America
OCIS Codes
(110.5120) Imaging systems : Photoacoustic imaging
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.3650) Medical optics and biotechnology : Lifetime-based sensing
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.1065) Medical optics and biotechnology : Acousto-optics
(170.2655) Medical optics and biotechnology : Functional monitoring and imaging
ToC Category:
Imaging Systems
History
Original Manuscript: November 1, 2010
Revised Manuscript: December 20, 2010
Manuscript Accepted: December 21, 2010
Published: March 1, 2011
Virtual Issues
Vol. 6, Iss. 4 Virtual Journal for Biomedical Optics
Citation
Amos Danielli, Christopher P. Favazza, Konstantin Maslov, and Lihong V. Wang, "Single-wavelength functional photoacoustic microscopy in biological tissue," Opt. Lett. 36, 769-771 (2011)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-36-5-769
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