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  • Vol. 36, Iss. 6 — Mar. 15, 2011
  • pp: 912–914

Dual-wavelength linear regression phase unwrapping in three-dimensional microscopic images of cancer cells

Alexander Khmaladze, Rebecca L. Matz, Chi Zhang, Ting Wang, Mark M. Banaszak Holl, and Zhan Chen  »View Author Affiliations


Optics Letters, Vol. 36, Issue 6, pp. 912-914 (2011)
http://dx.doi.org/10.1364/OL.36.000912


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Abstract

We present a study of the three-dimensional structure of cancer cells using dual-wavelength phase-imaging digital holographic microscopy. Phase imaging of objects with optical height variation greater than the wavelength of light is ambiguous and causes phase wrapping. By comparing two phase images recorded at different wavelengths, the images can be accurately unwrapped. The unwrapping method is computationally fast and straightforward, and it can process complex topologies. Additionally, the limitations on the total optical height are significantly relaxed. This new methodology is widely applicable to other phase-imaging techniques as well as in applications beyond optical microscopy.

© 2011 Optical Society of America

OCIS Codes
(170.0110) Medical optics and biotechnology : Imaging systems
(180.6900) Microscopy : Three-dimensional microscopy
(090.1995) Holography : Digital holography
(100.5088) Image processing : Phase unwrapping

ToC Category:
Holography

History
Original Manuscript: October 28, 2010
Revised Manuscript: February 14, 2011
Manuscript Accepted: February 15, 2011
Published: March 10, 2011

Virtual Issues
Vol. 6, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Alexander Khmaladze, Rebecca L. Matz, Chi Zhang, Ting Wang, Mark M. Banaszak Holl, and Zhan Chen, "Dual-wavelength linear regression phase unwrapping in three-dimensional microscopic images of cancer cells," Opt. Lett. 36, 912-914 (2011)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-36-6-912


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