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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 37, Iss. 1 — Jan. 1, 2012
  • pp: 22–24

Fabrication of optical mosaics mimicking human corneal endothelium for the training and assessment of eye bank technicians

Manuel Flury, Zhiguo He, Nelly Campolmi, Philippe Gain, Bernard Kress, and Gilles Thuret  »View Author Affiliations

Optics Letters, Vol. 37, Issue 1, pp. 22-24 (2012)

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The determination of endothelial cell density (ECD) is a crucial activity in eye banks for the assessment of corneal tissue quality. These cells are responsible for corneal transparency, and ECD correlates with graft survival. ECD is mainly assessed with a manual “naked-eye” procedure under a transmitted light microscope in Europe and using a specular microscope in the United States. Interbank and intrabank variability has been previously demonstrated. In order to facilitate training and continuing education of technicians and reliability assessment of eye banks’ ECD determination, we use micro-optics technologies to fabricate test mosaics that exactly reproduce the image of human corneal endothelium. The description of the fabrication process is detailed, and comparisons are made between amplitude and phase mosaics.

© 2012 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.4470) Medical optics and biotechnology : Ophthalmology
(220.4000) Optical design and fabrication : Microstructure fabrication

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: July 20, 2011
Revised Manuscript: November 14, 2011
Manuscript Accepted: November 14, 2011
Published: December 22, 2011

Virtual Issues
Vol. 7, Iss. 3 Virtual Journal for Biomedical Optics

Manuel Flury, Zhiguo He, Nelly Campolmi, Philippe Gain, Bernard Kress, and Gilles Thuret, "Fabrication of optical mosaics mimicking human corneal endothelium for the training and assessment of eye bank technicians," Opt. Lett. 37, 22-24 (2012)

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