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Targeted alteration of real and imaginary refractive index of biological cells by histological staining |
Optics Letters, Vol. 37, Issue 10, pp. 1601-1603 (2012)
http://dx.doi.org/10.1364/OL.37.001601
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Abstract
Various staining techniques are commonly used in biomedical research to investigate cellular morphology. By inducing absorption of light, staining dyes change the intracellular refractive index due to the Kramers–Kronig relationship. We present a method for creating 2D maps of real and imaginary refractive indices of stained biological cells using their thickness and absorptance. We validate our technique on dyed polystyrene microspheres and quantify the alteration in refractive index of stained biological cells. We reveal that specific staining of individual organelles can increase their scattering cross-section by orders of magnitudes, implying a major impact in the field of biophotonics.
© 2012 Optical Society of America
OCIS Codes
(160.4760) Materials : Optical properties
(170.0180) Medical optics and biotechnology : Microscopy
(170.1530) Medical optics and biotechnology : Cell analysis
(290.0290) Scattering : Scattering
(300.6550) Spectroscopy : Spectroscopy, visible
ToC Category:
Materials
History
Original Manuscript: December 2, 2011
Revised Manuscript: January 27, 2012
Manuscript Accepted: February 11, 2012
Published: May 9, 2012
Virtual Issues
Vol. 7, Iss. 7 Virtual Journal for Biomedical Optics
Citation
L. Cherkezyan, H. Subramanian, V. Stoyneva, J. D. Rogers, S. Yang, D. Damania, A. Taflove, and V. Backman, "Targeted alteration of real and imaginary refractive index of biological cells by histological staining," Opt. Lett. 37, 1601-1603 (2012)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-37-10-1601
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