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Simultaneous optically sectioned fluorescence and optical coherence microscopy with full-field illumination |
Optics Letters, Vol. 37, Issue 10, pp. 1613-1615 (2012)
http://dx.doi.org/10.1364/OL.37.001613
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Abstract
Full-field optical coherence microscopy (FF-OCM) and optically sectioned fluorescence microscopy are two imaging techniques that are implemented here in a novel dual modality instrument. The two imaging modalities use a broad field illumination to acquire the entire field of view without raster scanning. Optical sectioning is achieved in both imaging modalities owing to the coherence gating property of light for FF-OCM, and a structured illumination setup for fluorescence microscopy. Complementary image data are provided by the dual modality instrument in the context of biological tissue screening. FF-OCM imaging modality shows the tissue microarchitecture, while fluorescence microscopy highlights specific tissue features with cellular-level resolution by using targeting contrast agents. Complementary tissue morphology and biochemical features could potentially improve the understanding of cellular functions and disease diagnosis.
© 2012 Optical Society of America
OCIS Codes
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(170.4500) Medical optics and biotechnology : Optical coherence tomography
(180.3170) Microscopy : Interference microscopy
(170.2945) Medical optics and biotechnology : Illumination design
ToC Category:
Medical Optics and Biotechnology
History
Original Manuscript: January 23, 2012
Manuscript Accepted: March 1, 2012
Published: May 7, 2012
Virtual Issues
Vol. 7, Iss. 7 Virtual Journal for Biomedical Optics
May 4, 2012 Spotlight on Optics
Citation
Houssine Makhlouf, Karen Perronet, Guillaume Dupuis, Sandrine Lévêque-Fort, and Arnaud Dubois, "Simultaneous optically sectioned fluorescence and optical coherence microscopy
with full-field illumination," Opt. Lett. 37, 1613-1615 (2012)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-37-10-1613
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