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Optics Letters


  • Editor: Alan E. Willner
  • Vol. 37, Iss. 11 — Jun. 1, 2012
  • pp: 1805–1807

Quantitative pupil analysis in stimulated emission depletion microscopy using phase retrieval

Emil B. Kromann, Travis J. Gould, Manuel F. Juette, Jens E. Wilhjelm, and Joerg Bewersdorf  »View Author Affiliations

Optics Letters, Vol. 37, Issue 11, pp. 1805-1807 (2012)

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The resolution attainable with stimulated emission depletion (STED) microscopy greatly depends on the quality of the STED laser focus. So far, visual inspection of a measured STED focus has been the only convenient means of gauging the source of aberrations. Here we describe a method, requiring no instrument modifications, for obtaining an equivalent to the complex pupil function at the back aperture of the objective and show that it provides quantitative information about aberration sources (including aberrations induced by the objective or sample). We show the accuracy of this field representation to be sufficient for reconstructing the STED focus in three dimensions and determining corrective steps.

© 2012 Optical Society of America

OCIS Codes
(100.5070) Image processing : Phase retrieval
(100.6640) Image processing : Superresolution
(170.5810) Medical optics and biotechnology : Scanning microscopy
(080.4865) Geometric optics : Optical vortices

ToC Category:
Image Processing

Original Manuscript: February 14, 2012
Manuscript Accepted: February 22, 2012
Published: May 17, 2012

Virtual Issues
Vol. 7, Iss. 8 Virtual Journal for Biomedical Optics

Emil B. Kromann, Travis J. Gould, Manuel F. Juette, Jens E. Wilhjelm, and Joerg Bewersdorf, "Quantitative pupil analysis in stimulated emission depletion microscopy using phase retrieval," Opt. Lett. 37, 1805-1807 (2012)

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  1. S. W. Hell, Nat. Meth. 6, 24 (2009). [CrossRef]
  2. D. Toomre and J. Bewersdorf, Annu. Rev. Cell Dev. Biol. 26, 285 (2010). [CrossRef]
  3. S. W. Hell and J. Wichmann, Opt. Lett. 19, 780 (1994). [CrossRef]
  4. E. Rittweger, K. Y. Han, S. E. Irvine, C. Eggeling, and S. W. Hell, Nat. Photon. 3, 144 (2009). [CrossRef]
  5. S. Deng, L. Liu, Y. Cheng, R. Li, and Z. Xu, Opt. Express 17, 1714 (2009). [CrossRef]
  6. L. Kastrup, D. Wildanger, B. R. Rankin, and S. W. Hell, in Nanoscopy and Multidimensional Optical Fluorescence Microscopy, A. Diaspro, ed. (Chapman & Hall/CRC, 2010), pp. 1–13.
  7. B. M. Hanser, M. G. Gustafsson, D. A. Agard, and J. W. Sedat, J. Microsc. 216, 32 (2004). [CrossRef]
  8. T. J. Gould, J. R. Myers, and J. Bewersdorf, Opt. Express 19, 13351 (2011). [CrossRef]
  9. D. Débarre, T. Vieille, and E. Beaurepaire, J. Microsc. 244, 136 (2011). [CrossRef]
  10. T. Züchner, A. V. Failla, and A. J. Meixner, Angew. Chem. 50, 5274 (2011). [CrossRef]

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