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Optics Letters

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  • Editor: Alan E. Willner
  • Vol. 37, Iss. 11 — Jun. 1, 2012
  • pp: 1817–1819

A spectrally constrained dual-band normalization technique for protoporphyrin IX quantification in fluorescence-guided surgery

P. A. Valdés, F. Leblond, A. Kim, B. C. Wilson, K. D. Paulsen, and D. W. Roberts  »View Author Affiliations


Optics Letters, Vol. 37, Issue 11, pp. 1817-1819 (2012)
http://dx.doi.org/10.1364/OL.37.001817


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Abstract

We report a dual-band normalization technique for in vivo quantification of the metabolic biomarker, protoporphyrin IX (PpIX), during brain tumor resection procedures. The accuracy of the approach was optimized in tissue simulating phantoms with varying absorption and scattering properties, validated with fluorimetric assessments on ex vivo brain tissue, and tested on human data acquired in vivo during fluorescence-guided surgery of brain tumors. The results demonstrate that the dual-band normalization technique allows PpIX concentrations to be accurately quantified by correction with reflectance data recorded and integrated within only two narrow wavelength intervals. The simplicity of the method lends itself to the enticing prospect that the method could be applicable to wide-field applications in quantitative fluorescence imaging and dosimetry in photodynamic therapy.

© 2012 Optical Society of America

OCIS Codes
(170.1610) Medical optics and biotechnology : Clinical applications
(170.3660) Medical optics and biotechnology : Light propagation in tissues
(170.3890) Medical optics and biotechnology : Medical optics instrumentation
(170.6280) Medical optics and biotechnology : Spectroscopy, fluorescence and luminescence
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: February 1, 2012
Revised Manuscript: March 25, 2012
Manuscript Accepted: March 27, 2012
Published: May 17, 2012

Virtual Issues
Vol. 7, Iss. 8 Virtual Journal for Biomedical Optics

Citation
P. A. Valdés, F. Leblond, A. Kim, B. C. Wilson, K. D. Paulsen, and D. W. Roberts, "A spectrally constrained dual-band normalization technique for protoporphyrin IX quantification in fluorescence-guided surgery," Opt. Lett. 37, 1817-1819 (2012)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-37-11-1817


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References

  1. P. A. Valdes, F. Leblond, A. Kim, B. T. Harris, B. C. Wilson, X. Fan, T. D. Tosteson, A. Hartov, S. Ji, K. Erkmen, N. E. Simmons, K. D. Paulsen, and D. W. Roberts, J. Neurosurg. 115, 11 (2011). [CrossRef]
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  5. A. Kim, M. Khurana, Y. Moriyama, and B. C. Wilson, J. Biomed. Opt. 15, 067006 (2010). [CrossRef]
  6. L. Lilge, C. O’Carroll, and B. C. Wilson, J. Photochem. Photobiol. B 39, 229 (1997). [CrossRef]

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