OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Vol. 37, Iss. 13 — Jul. 1, 2012
  • pp: 2445–2447

Fluorescence holography with improved signal-to-noise ratio by near image plane recording

Xiaomin Lai, Yuan Zhao, Xiaohua Lv, Zhenqiao Zhou, and Shaoqun Zeng  »View Author Affiliations


Optics Letters, Vol. 37, Issue 13, pp. 2445-2447 (2012)
http://dx.doi.org/10.1364/OL.37.002445


View Full Text Article

Enhanced HTML    Acrobat PDF (381 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

Holographic fluorescence imaging is very promising, as it can obtain three-dimensional fluorescence imaging without scanning. However, the current method usually records holograms far from the image plane, with the fluorescence decaying when spreading broadly. Here we show that the signal-to-noise ratio (SNR) of fluorescence holography can be improved by recording the high-contrast interferogram near the image plane. We found that this can be achieved by setting the focal length of the lens for the reference wave (f2) close to that for the object wave (f1). With experiments, we demonstrate an example of an increase of about 21 times in SNR by changing f2 from infinity to 226 mm, which is close to f1 (323 mm).

© 2012 Optical Society of America

OCIS Codes
(090.2880) Holography : Holographic interferometry
(110.6880) Imaging systems : Three-dimensional image acquisition
(170.2520) Medical optics and biotechnology : Fluorescence microscopy
(090.1995) Holography : Digital holography

ToC Category:
Holography

History
Original Manuscript: March 12, 2012
Revised Manuscript: April 9, 2012
Manuscript Accepted: April 19, 2012
Published: June 18, 2012

Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Xiaomin Lai, Yuan Zhao, Xiaohua Lv, Zhenqiao Zhou, and Shaoqun Zeng, "Fluorescence holography with improved signal-to-noise ratio by near image plane recording," Opt. Lett. 37, 2445-2447 (2012)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-37-13-2445


Sort:  Author  |  Year  |  Journal  |  Reset  

References

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1. Fig. 2. Fig. 3.
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited