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Optics Letters

Optics Letters


  • Vol. 37, Iss. 13 — Jul. 1, 2012
  • pp: 2481–2483

Fast and precise algorithm based on maximum radial symmetry for single molecule localization

Hongqiang Ma, Fan Long, Shaoqun Zeng, and Zhen-Li Huang  »View Author Affiliations

Optics Letters, Vol. 37, Issue 13, pp. 2481-2483 (2012)

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We present an algorithm to estimate the location of single fluorescent molecule with both high speed and high precision. This algorithm is based on finding the subpixel position with maximum radial symmetry in a pixelated single molecule fluorescence image. Compared with conventional algorithms, this algorithm does not rely on point-spread-function or noise model. Through numerical simulation and experimental analysis, we found that this algorithm exhibits localization precision very close to the maximum likelihood estimator (MLE), while executes 1000 times faster than the MLE and 6 times faster than the fluoroBancroft algorithm.

© 2012 Optical Society of America

OCIS Codes
(100.6640) Image processing : Superresolution
(110.2960) Imaging systems : Image analysis
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:

Original Manuscript: March 2, 2012
Revised Manuscript: March 26, 2012
Manuscript Accepted: April 25, 2012
Published: June 19, 2012

Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics

Hongqiang Ma, Fan Long, Shaoqun Zeng, and Zhen-Li Huang, "Fast and precise algorithm based on maximum radial symmetry for single molecule localization," Opt. Lett. 37, 2481-2483 (2012)

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  1. B. Huang, H. Babcock, and X. W. Zhuang, Cell 143, 1047 (2010). [CrossRef]
  2. R. J. Ober, S. Ram, and E. S. Ward, Biophys. J. 86, 1185 (2004). [CrossRef]
  3. Z. L. Shen and S. B. Andersson, IEEE Trans. Signal Process. 59, 4041 (2011). [CrossRef]
  4. B. Yu, D. N. Chen, J. L. Qu, and H. B. Niu, Opt. Lett. 36, 4317 (2011). [CrossRef]
  5. R. Starr, S. Stahlheber, and A. Small, Opt. Lett. 37, 413 (2012). [CrossRef]
  6. T. W. Quan, P. C. Li, F. Long, S. Q. Zeng, Q. M. Luo, P. N. Hedde, G. U. Nienhaus, and Z. L. Huang, Opt. Express 18, 11867 (2010). [CrossRef]
  7. C. S. Smith, N. Joseph, B. Rieger, and K. A. Lidke, Nat. Meth. 7, 373 (2010). [CrossRef]
  8. Z. L. Huang, H. Y. Zhu, F. Long, H. Q. Ma, L. S. Qin, Y. F. Liu, J. P. Ding, Z. H. Zhang, Q. M. Luo, and S. Q. Zeng, Opt. Express 19, 19156 (2011). [CrossRef]
  9. P. Friedl and D. Gilmour, Nat. Rev. Mol. Cell Biol. 10, 445 (2009). [CrossRef]
  10. J. B. Pawley, Handbook of Biological Confocal Microscopy, 3rd ed. (Springer, 2006).
  11. P. N. Hedde, J. Fuchs, F. Oswald, J. Wiedenmann, and G. U. Nienhaus, Nat. Meth. 6, 689 (2009). [CrossRef]
  12. K. Nienhaus, G. U. Nienhaus, J. Wiedenmann, and H. Nar, Proc. Natl. Acad. Sci. USA 102, 9156 (2005). [CrossRef]

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