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Optics Letters

Optics Letters


  • Vol. 37, Iss. 13 — Jul. 1, 2012
  • pp: 2490–2492

Label-free in vivo flow cytometry in zebrafish using two-photon autofluorescence imaging

Yan Zeng, Jin Xu, Dong Li, Li Li, Zilong Wen, and Jianan Y. Qu  »View Author Affiliations

Optics Letters, Vol. 37, Issue 13, pp. 2490-2492 (2012)

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We demonstrate a label-free in vivo flow cytometry in zebrafish blood vessels based on two-photon excited autofluorescence imaging. The major discovery in this work is the strong autofluorescence emission from the plasma in zebrafish blood. The plasma autofluorescence provides excellent contrast for visualizing blood vessels and counting blood cells. In addition, the cellular nicotinamide adenine dinucleotide autofluorescence enables in vivo imaging and counting of white blood cells (neutrophils).

© 2012 Optical Society of America

OCIS Codes
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(180.5810) Microscopy : Scanning microscopy
(180.4315) Microscopy : Nonlinear microscopy
(170.6935) Medical optics and biotechnology : Tissue characterization

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: February 13, 2012
Revised Manuscript: April 9, 2012
Manuscript Accepted: May 7, 2012
Published: June 20, 2012

Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics

Yan Zeng, Jin Xu, Dong Li, Li Li, Zilong Wen, and Jianan Y. Qu, "Label-free in vivo flow cytometry in zebrafish using two-photon autofluorescence imaging," Opt. Lett. 37, 2490-2492 (2012)

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  1. V. V. Tuchin, A. Tárnok, and V. P. Zharov, Cytom. A 79, 737 (2011). [CrossRef]
  2. J. Novak, I. Georgakoudi, X. Wei, A. Prossin, and C. P. Lin, Opt. Lett. 29, 77 (2004). [CrossRef]
  3. C. F. Zhong, E. R. Tkaczyk, T. Thomas, J. Y. Ye, A. Myc, A. U. Bielinska, Z. Cao, I. Majoros, B. Keszler, J. R. Baker, and T. B. Norris, J. Biomed. Opt. 13, 034008 (2008). [CrossRef]
  4. G. A. Wagnières, W. M. Star, and B. C. Wilson, Photochem. Photobiol. 68, 603 (1998). [CrossRef]
  5. L. V. Wang, Nat. Photon. 3, 503 (2009). [CrossRef]
  6. W. Zheng, D. Li, Y. Zeng, Y. Luo, and J. Y. Qu, Biomed. Opt. Express 2, 71 (2011). [CrossRef]
  7. D. Li, W. Zheng, and J. Y. Qu, Opt. Lett. 34, 2853 (2009). [CrossRef]
  8. G. J. Lieschke, and P. D. Currie, Nat. Rev. Genet. 8, 353 (2007). [CrossRef]
  9. D. F. Edwards, The Zebrafish Book: A Guide for the Laboratory Use of Zebrafish (Danio rerio), 3rd ed.(University of Oregon, 1995).
  10. W. Zheng, Y. Wu, D. Li, and J. Y. Qu, J. Biomed. Opt. 13, 054010 (2008). [CrossRef]
  11. E. I. Galanzha and V. P. Zharov, Cytom. A 79, 746 (2011). [CrossRef]
  12. K. Ley, C. Laudanna, M. I. Cybulsky, and S. Nourshargh, Nat. Rev. Immunol. 7, 678 (2007). [CrossRef]
  13. W. R. Zipfel, R. M. Williams, R. Christiet, A. Y. Nikitin, B. T. Hyman, and W. W. Webb, Proc. Natl. Acad. Sci. USA 100, 7075 (2003). [CrossRef]

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