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  • Vol. 37, Iss. 13 — Jul. 1, 2012
  • pp: 2625–2627

Validation of the protoporphyrin IX–triplet state lifetime technique for mitochondrial oxygen measurements in the skin

Floor A. Harms, Sander I. A. Bodmer, Nicolaas J. H. Raat, Robert J. Stolker, and Egbert G. Mik  »View Author Affiliations


Optics Letters, Vol. 37, Issue 13, pp. 2625-2627 (2012)
http://dx.doi.org/10.1364/OL.37.002625


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Abstract

Mitochondrial oxygen tension can be measured in vivo by means of oxygen-dependent quenching of delayed fluorescence of protoporphyrin IX (PpIX). Here we demonstrate that mitochondrial PO2 (mitoPO2) can be measured in the skin of a rat after topical application of the PpIX precursor 5-aminolevulinic acid (ALA). Calibration of mitoPO2 measurements was done by comparison with simultaneous measurements of the cutaneous microvascular PO2 This was done under three different conditions: in normal skin tissue, in nonrespiration skin tissue due to the application of cyanide, and in anoxic skin tissue after the ventilation with 100% nitrogen. The results of this study show that it is feasible to measure the mitoPO2 after the topical application of ALA cream by means of the PpIX–triplet state lifetime technique.

© 2012 Optical Society of America

OCIS Codes
(170.1470) Medical optics and biotechnology : Blood or tissue constituent monitoring
(170.3650) Medical optics and biotechnology : Lifetime-based sensing

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: March 8, 2012
Manuscript Accepted: April 18, 2012
Published: June 25, 2012

Virtual Issues
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics

Citation
Floor A. Harms, Sander I. A. Bodmer, Nicolaas J. H. Raat, Robert J. Stolker, and Egbert G. Mik, "Validation of the protoporphyrin IX–triplet state lifetime technique for mitochondrial oxygen measurements in the skin," Opt. Lett. 37, 2625-2627 (2012)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-37-13-2625


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