Homodyne detection can dramatically enhance measurement sensitivity for weak signals. In nonlinear optical microscopy it can make accessible a range of novel, intrinsic, contrast like nonlinear absorption and nonlinear phase contrast. Here a compact and rapid pulse shaper is developed, implemented, and demonstrated for homodyne detection in nonlinear microscopy with high-repetition rate mode-locked femtosecond lasers. With this method we generate two-photon absorption (TPA) and self-phase modulation images of gold nanostars in biological samples. Simultaneous imaging of two-photon luminescence and TPA also enables us to produce two-photon quantum yield images.
© 2012 Optical Society of America
Original Manuscript: February 28, 2012
Manuscript Accepted: April 25, 2012
Published: June 29, 2012
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics
Baolei Li, Kevin E. Claytor, Hsiangkuo Yuan, Tuan Vo-Dinh, Warren S. Warren, and Martin C. Fischer, "Multicontrast nonlinear optical microscopy with a compact and rapid pulse shaper," Opt. Lett. 37, 2763-2765 (2012)