A simple technique for remote scanning of the focal plane in temporal focusing multiphoton microscopy is demonstrated both theoretically and experimentally. A new on-axis light propagation optical setup design enables this scanning, which was considered not feasible in previous studies. The focal plane is axially displaced by the movement of a remote optical device, consisting of a double prism grating, and optionally a cylindrical lens. The displacement is linear, and its slope is inversely proportional to the square of the optical system’s magnification.
© 2012 Optical Society of America
Original Manuscript: May 1, 2012
Revised Manuscript: May 17, 2012
Manuscript Accepted: June 4, 2012
Published: July 12, 2012
Vol. 7, Iss. 9 Virtual Journal for Biomedical Optics
Hod Dana and Shy Shoham, "Remotely scanned multiphoton temporal focusing by axial grism scanning," Opt. Lett. 37, 2913-2915 (2012)