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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 37, Iss. 16 — Aug. 15, 2012
  • pp: 3312–3314

Quantification of lamellar orientation in corneal collagen using second harmonic generation images

Yair Mega, Mike Robitaille, Ramin Zareian, James McLean, Jeffrey Ruberti, and Charles DiMarzio  »View Author Affiliations

Optics Letters, Vol. 37, Issue 16, pp. 3312-3314 (2012)

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Second harmonic generation (SHG) is a well-established optical modality widely used in biomedical optics to image collagen based tissues. The coherent signal of the forward direction SHG produces a high resolution image that can resolve individual fibers (groups of fibrils). In highly ordered collagen lamellae, such as in the corneal stroma, it is important to determine the orientation of the fibers as they contribute significantly to the biomechanics of the tissue. However, due to the crimped structure of the fibers, it is challenging to robustly determine their orientation using an independent computational method, compared to the straight fibers problem. Previous work in the field used the polarization of the fundamental or other techniques involving a more manual selection of the orientation, in order to differentiate between various directions in corneal structures. Yet those lack accuracy and independency. We present a robust independent technique to determine the orientation of the fibers in the corneal structure. The experimental results presented here, taken from different lamellae, demonstrate strongly the correct orientation.

© 2012 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(110.1758) Imaging systems : Computational imaging

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: June 12, 2012
Revised Manuscript: June 27, 2012
Manuscript Accepted: June 27, 2012
Published: August 3, 2012

Virtual Issues
Vol. 7, Iss. 10 Virtual Journal for Biomedical Optics

Yair Mega, Mike Robitaille, Ramin Zareian, James McLean, Jeffrey Ruberti, and Charles DiMarzio, "Quantification of lamellar orientation in corneal collagen using second harmonic generation images," Opt. Lett. 37, 3312-3314 (2012)

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