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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 37, Iss. 17 — Sep. 1, 2012
  • pp: 3630–3632

Autofocusing of digital holographic microscopy based on off-axis illuminations

Peng Gao, Baoli Yao, Junwei Min, Rongli Guo, Baiheng Ma, Juanjuan Zheng, Ming Lei, Shaohui Yan, Dan Dan, and Tong Ye  »View Author Affiliations

Optics Letters, Vol. 37, Issue 17, pp. 3630-3632 (2012)

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An auto-focusing method for digital holographic microscopy has been proposed by employing two off-axis illumination beams. When specimens are illuminated by two plane waves in different directions, it is found that the farther the reconstruction plane is from the image plane, the wider the two reconstructed images are separated from each other. Thus, the image plane can be determinated by finding the minimum of the variation between the two reconstructed object waves on both the amplitude and phase distributions. The feasibility of the proposed method is demonstrated by the corresponding simulation and experiment.

© 2012 Optical Society of America

OCIS Codes
(090.2880) Holography : Holographic interferometry
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(180.3170) Microscopy : Interference microscopy
(090.1995) Holography : Digital holography

ToC Category:

Original Manuscript: April 27, 2012
Revised Manuscript: July 1, 2012
Manuscript Accepted: July 12, 2012
Published: August 27, 2012

Virtual Issues
Vol. 7, Iss. 11 Virtual Journal for Biomedical Optics

Peng Gao, Baoli Yao, Junwei Min, Rongli Guo, Baiheng Ma, Juanjuan Zheng, Ming Lei, Shaohui Yan, Dan Dan, and Tong Ye, "Autofocusing of digital holographic microscopy based on off-axis illuminations," Opt. Lett. 37, 3630-3632 (2012)

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