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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 37, Iss. 18 — Sep. 15, 2012
  • pp: 3837–3839

Second-harmonic generation circular dichroism studies of osteogenesis imperfecta

Xiyi Chen, Cathleen Raggio, and Paul J. Campagnola  »View Author Affiliations

Optics Letters, Vol. 37, Issue 18, pp. 3837-3839 (2012)

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We report the use of second-harmonic generation (SHG) microscopy in conjunction with circular dichroism (CD) to differentiate normal skin from that in the connective tissue disorder osteogenesis imperfecta (OI). Osteogenesis imperfecta results from mutations in the collagen triple helix, where the individual chains are defective, leading to abnormal folding, and ultimately, abnormal fibril/fiber organization. Second-harmonic-generation circular dichroism successfully differentiated normal human and OI skin tissues, whereas other SHG polarization schemes did not provide discrimination, suggesting this approach has high sensitivity for studying the difference in chirality in the mutated collagen. We further suggest that the method has clinical diagnostic value, as it could be performed with minimal invasion.

© 2012 Optical Society of America

OCIS Codes
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(170.6930) Medical optics and biotechnology : Tissue
(180.6900) Microscopy : Three-dimensional microscopy
(190.2620) Nonlinear optics : Harmonic generation and mixing

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: July 5, 2012
Manuscript Accepted: July 27, 2012
Published: September 11, 2012

Virtual Issues
Vol. 7, Iss. 11 Virtual Journal for Biomedical Optics

Xiyi Chen, Cathleen Raggio, and Paul J. Campagnola, "Second-harmonic generation circular dichroism studies of osteogenesis imperfecta," Opt. Lett. 37, 3837-3839 (2012)

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