A simple and practical method for measuring the angle-resolved light scattering (ARLS) from individual objects is reported. Employing the principle of inline holography and a Fourier transform light scattering technique, both the static and dynamic scattering patterns from individual micrometer-sized objects can be effectively and quantitatively obtained. First, the light scattering measurements were performed on individual polystyrene beads, from which the refractive index and diameter of each bead were retrieved. Also, the measurements of the static and dynamic light scattering from intact human red blood cells are demonstrated. Using the present method, an existing microscope can be directly transformed into a precise instrument for ARLS measurements.
© 2012 Optical Society of America
Original Manuscript: June 7, 2012
Revised Manuscript: August 18, 2012
Manuscript Accepted: September 4, 2012
Published: September 28, 2012
Kyoohyun Kim and YongKeun Park, "Fourier transform light scattering angular spectroscopy using digital inline holography," Opt. Lett. 37, 4161-4163 (2012)