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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 37, Iss. 20 — Oct. 15, 2012
  • pp: 4173–4175

Precision increase with two orthogonal analyzers in polarization-resolved second-harmonic generation microscopy

Philippe Réfrégier, Muriel Roche, Julien Duboisset, and Sophie Brasselet  »View Author Affiliations

Optics Letters, Vol. 37, Issue 20, pp. 4173-4175 (2012)

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We analyze the increase in precision of parameters estimation for polarization-resolved second-harmonic generation imaging microscopy when two intensities are measured with two orthogonal analyzers. The analysis is performed for measuring anisotropy parameters and molecule orientation for samples with cylindrical symmetry in the presence of photon noise with Poisson statistics. The improvement in comparison to global intensity measurement (i.e., without analyzer) is discussed.

© 2012 Optical Society of America

OCIS Codes
(030.6600) Coherence and statistical optics : Statistical optics
(110.4280) Imaging systems : Noise in imaging systems
(190.1900) Nonlinear optics : Diagnostic applications of nonlinear optics
(180.4315) Microscopy : Nonlinear microscopy
(110.5405) Imaging systems : Polarimetric imaging

ToC Category:
Imaging Systems

Original Manuscript: July 18, 2012
Manuscript Accepted: August 27, 2012
Published: October 2, 2012

Philippe Réfrégier, Muriel Roche, Julien Duboisset, and Sophie Brasselet, "Precision increase with two orthogonal analyzers in polarization-resolved second-harmonic generation microscopy," Opt. Lett. 37, 4173-4175 (2012)

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  1. I. Freund, M. Deutsch, and A. Sprecher, Biophys. J. 50, 693 (1986). [CrossRef]
  2. P. Stoller, B. M. Kim, A. M. Rubenchik, K. M. Reiser, and L. B. Da Silva, J. Biomed. Opt. 7, 205 (2000). [CrossRef]
  3. T. Yasui, K. Sasaki, Y. Tohno, and T. Araki, Opt. Quantum Electron. 37, 1397 (2005). [CrossRef]
  4. R. M. Williams, W. R. Zipfel, and W. W. Webb, Biophys. J. 88, 1377 (2005). [CrossRef]
  5. S. V. Plotnikov, A. Millard, P. Campagnola, and W. Mohler, Biophys. J. 90, 328 (2006). [CrossRef]
  6. F. Tiaho, G. Recher, and D. Rouède, Opt. Express 15, 4054 (2007). [CrossRef]
  7. W.-L. Chen, T.-H. Li, P.-J. Su, C.-K. Chou, P. T. Fwu, S.-J. Lin, D. Kim, P. T. C. So, and C.-Y. Dong, Appl. Phys. Lett. 94, 183902 (2009). [CrossRef]
  8. S. Psilodimitrakopoulos, S. I. C. O. Santos, I. Amat-Roldan, A. K. N. Thayil, D. Artigas, and P. Loza-Alvarez, J. Biomed. Opt. 14, 014001 (2009). [CrossRef]
  9. D. Aït-Belkacem, A. Gasecka, F. Munhoz, S. Brustlein, and S. Brasselet, Opt. Express 18, 14859 (2010). [CrossRef]
  10. I. Gusachenko, Y. G. Houssen, V. Tran, J.-M. Allain, and M.-C. Schanne-Klein, Biophys. J. 102, 2220 (2012). [CrossRef]
  11. P. J. Su, W. L. Chen, Y. F. Chen, and C. Y. Dong, Biophys. J. 100, 2053 (2011). [CrossRef]
  12. Ph. Réfrégier, M. Roche, and S. Brasselet, Opt. Lett. 36, 2149 (2011). [CrossRef]
  13. V. Le Floc’h, S. Brasselet, J.-F. Roch, and J. Zyss, J. Phys. Chem. B 107, 12403 (2003). [CrossRef]
  14. S. Brasselet, V. Le Floc’h, F. Treussart, J.-F. Roch, and J. Zyss, Phys. Rev. Lett. 92, 207401 (2004). [CrossRef]
  15. J. Duboisset, D. Aït-Belkacem, M. Roche, H. Rigneault, and S. Brasselet, Phys. Rev. A 85, 043829 (2012). [CrossRef]
  16. H. V. Poor, An Introduction to Signal Detection and Estimation (Springer-Verlag, 1998).

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