Laboratory water window cryomicroscopy has recently demonstrated similar image quality as synchrotron-based microscopy but still with much longer exposure times, prohibiting the spread to a wider scientific community. Here we demonstrate high-resolution laboratory water window imaging of cryofrozen cells with 10 s range exposure times. The major improvement is the operation of a $\lambda =2.48\mathrm{nm}$, 2 kHz liquid nitrogen jet laser plasma source with high spatial and temporal stability at high average brightness $>1.5\times {10}^{12}\mathrm{ph}/(\mathrm{s}\times \mathrm{sr}\times {\mathrm{\mu m}}^2\times \text{line})$, i.e., close to that of early synchrotrons. Thus, this source enables not only biological x-ray microscopy in the home laboratory but potentially other applications previously only accessible at synchrotron facilities.

© 2012 Optical Society of America

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