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Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 37, Iss. 21 — Nov. 1, 2012
  • pp: 4495–4497

Assessing the tissue-imaging performance of confocal microscope architectures via Monte Carlo simulations

Ye Chen, Danni Wang, and Jonathan T. C. Liu  »View Author Affiliations


Optics Letters, Vol. 37, Issue 21, pp. 4495-4497 (2012)
http://dx.doi.org/10.1364/OL.37.004495


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Abstract

Various confocal microscope architectures have been developed for in vivo tissue imaging, including single-axis confocal (SAC) and dual-axis confocal (DAC) configurations utilizing both point-scanning (PS) and line-scanning (LS) approaches. While it is known that these design variations lead to tradeoffs in imaging performance, a quantitative comparison of the imaging performance of these configurations in highly turbid media would be of value. Here, we perform Monte Carlo simulations to evaluate the optical-sectioning capability of these various confocal microscope architectures in reflectance mode. In particular, we investigate the axial and transverse responses of these configurations to reflective targets at various depths within a homogenous scattering medium. We find that the DAC-PS configuration results in superior rejection of multiply scattered background light compared to all other configurations, followed in performance by the SAC-PS, the DAC-LS, and then the SAC-LS. Line scanning with both the DAC and SAC configurations leads to photon crosstalk between pixels. However, at shallow depths, the axial and transverse resolution of all configurations is maintained in a homogeneous scattering medium.

© 2012 Optical Society of America

OCIS Codes
(170.0180) Medical optics and biotechnology : Microscopy
(170.1790) Medical optics and biotechnology : Confocal microscopy
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.5810) Medical optics and biotechnology : Scanning microscopy
(290.4210) Scattering : Multiple scattering
(110.0113) Imaging systems : Imaging through turbid media

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: August 3, 2012
Revised Manuscript: September 10, 2012
Manuscript Accepted: September 25, 2012
Published: October 26, 2012

Citation
Ye Chen, Danni Wang, and Jonathan T. C. Liu, "Assessing the tissue-imaging performance of confocal microscope architectures via Monte Carlo simulations," Opt. Lett. 37, 4495-4497 (2012)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-37-21-4495

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