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Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 37, Iss. 23 — Dec. 1, 2012
  • pp: 5018–5020

Wide and scalable field-of-view Talbot-grid-based fluorescence microscopy

Shuo Pang, Chao Han, Mihoko Kato, Paul W. Sternberg, and Changhuei Yang  »View Author Affiliations


Optics Letters, Vol. 37, Issue 23, pp. 5018-5020 (2012)
http://dx.doi.org/10.1364/OL.37.005018


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Abstract

Here we report a low-cost and simple wide field-of-view (FOV) on-chip fluorescence-imaging platform, termed fluorescence Talbot microscopy (FTM), which utilizes the Talbot self-imaging effect to enable efficient fluorescence imaging over a large and directly scalable FOV. The FTM prototype has a resolution of 1.2 μm and an FOV of 3.9mm×3.5mm. We demonstrate the imaging capability of FTM on fluorescently labeled breast cancer cells (SK-BR-3) and human embryonic kidney 293 (HEK) cells expressing green fluorescent protein.

© 2012 Optical Society of America

OCIS Codes
(110.6760) Imaging systems : Talbot and self-imaging effects
(180.2520) Microscopy : Fluorescence microscopy

ToC Category:
Microscopy

History
Original Manuscript: September 18, 2012
Revised Manuscript: October 24, 2012
Manuscript Accepted: November 5, 2012
Published: November 30, 2012

Virtual Issues
Vol. 8, Iss. 1 Virtual Journal for Biomedical Optics

Citation
Shuo Pang, Chao Han, Mihoko Kato, Paul W. Sternberg, and Changhuei Yang, "Wide and scalable field-of-view Talbot-grid-based fluorescence microscopy," Opt. Lett. 37, 5018-5020 (2012)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-37-23-5018

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