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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 37, Iss. 24 — Dec. 15, 2012
  • pp: 5127–5129

Quantitative phase-contrast imaging with compact digital holographic microscope employing Lloyd’s mirror

Vani Chhaniwal, Amardeep S. G. Singh, Rainer A. Leitgeb, Bahram Javidi, and Arun Anand  »View Author Affiliations

Optics Letters, Vol. 37, Issue 24, pp. 5127-5129 (2012)

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Digital holographic microscopy (DHM) is one of the most effective techniques used for quantitative phase imaging of cells. Here we present a compact, easy to implement, portable, and very stable DHM setup employing a self-referencing Lloyd’s mirror configuration. The microscope is constructed using a diode laser source and a CMOS sensor, making it cost effective. The reconstruction of recorded holograms yields the amplitude and phase information of the object. The temporal stability of the presented technique was found to be around 0.9 nm without any vibration compensation, which makes it ideal for studying cell profile changes. This aspect of the technique is demonstrated by studying membrane fluctuations of red blood cells.

© 2012 Optical Society of America

OCIS Codes
(120.3890) Instrumentation, measurement, and metrology : Medical optics instrumentation
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(140.2020) Lasers and laser optics : Diode lasers
(170.6900) Medical optics and biotechnology : Three-dimensional microscopy
(180.3170) Microscopy : Interference microscopy
(090.1995) Holography : Digital holography

ToC Category:

Original Manuscript: September 26, 2012
Manuscript Accepted: November 8, 2012
Published: December 10, 2012

Virtual Issues
Vol. 8, Iss. 1 Virtual Journal for Biomedical Optics

Vani Chhaniwal, Amardeep S. G. Singh, Rainer A. Leitgeb, Bahram Javidi, and Arun Anand, "Quantitative phase-contrast imaging with compact digital holographic microscope employing Lloyd’s mirror," Opt. Lett. 37, 5127-5129 (2012)

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