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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 37, Iss. 3 — Feb. 1, 2012
  • pp: 362–364

Integrated multimodal endomicroscopy platform for simultaneous en face optical coherence and two-photon fluorescence imaging

Jiefeng Xi, Yongping Chen, Yuying Zhang, Kartikeya Murari, Ming-Jun Li, and Xingde Li  »View Author Affiliations

Optics Letters, Vol. 37, Issue 3, pp. 362-364 (2012)

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We report an all-fiber-optic scanning, multimodal endomicroscope capable of simultaneous optical coherence tomography (OCT) and two-photon fluorescence (TPF) imaging. Both imaging modalities share the same miniature fiber-optic scanning endomicroscope, which consists of a double-clad fiber with a core operating in single mode at both the OCT (1310 nm) and two-photon excitation (1550 nm) wavelengths, a piezoelectric two-dimensional fiber-optic beam scanner, and a miniature aspherical compound lens suitable for simultaneous acquisition of en face OCT and TPF images. A fiber-optic wavelength division multiplexer was employed in the integrated platform to combine the low coherence OCT light source and the femtosecond two-photon excitation laser into the same optical path. Preliminary imaging results of cell cultures and mouse tissue ex vivo demonstrate the feasibility of simultaneous real-time OCT and TPF imaging in a scanning endomicroscopy setting for the first time.

© 2012 Optical Society of America

OCIS Codes
(110.4500) Imaging systems : Optical coherence tomography
(170.2150) Medical optics and biotechnology : Endoscopic imaging
(190.0190) Nonlinear optics : Nonlinear optics

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: September 30, 2011
Revised Manuscript: November 24, 2011
Manuscript Accepted: December 7, 2011
Published: January 20, 2012

Virtual Issues
Vol. 7, Iss. 4 Virtual Journal for Biomedical Optics

Jiefeng Xi, Yongping Chen, Yuying Zhang, Kartikeya Murari, Ming-Jun Li, and Xingde Li, "Integrated multimodal endomicroscopy platform for simultaneous en face optical coherence and two-photon fluorescence imaging," Opt. Lett. 37, 362-364 (2012)

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