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Oriented imaging of 3D subcellular structures in bacterial cells using optical tweezers |
Optics Letters, Vol. 37, Issue 3, pp. 440-442 (2012)
http://dx.doi.org/10.1364/OL.37.000440
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Abstract
Using oscillating optical tweezers, we show that controlled alignment of rod-shaped bacterial cells allows imaging fluorescently labeled three-dimensional (3D) subcellular structures from different, optimized viewpoints. To illustrate our method, we analyze the Z ring of E. coli. We obtain that the radial width of the Z ring in unconstricted cells is about 120 nm. This result suggests that the Z ring consists of an extremely sparse network of FtsZ filaments.
© 2012 Optical Society of America
OCIS Codes
(110.2960) Imaging systems : Image analysis
(180.6900) Microscopy : Three-dimensional microscopy
ToC Category:
Imaging Systems
History
Original Manuscript: September 15, 2011
Revised Manuscript: December 12, 2011
Manuscript Accepted: December 12, 2011
Published: February 1, 2012
Virtual Issues
Vol. 7, Iss. 4 Virtual Journal for Biomedical Optics
Citation
G. Carmon, I. Fishov, and M. Feingold, "Oriented imaging of 3D subcellular structures in bacterial cells using optical tweezers," Opt. Lett. 37, 440-442 (2012)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-37-3-440
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