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Optics Letters

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  • Editor: Alan E. Willner
  • Vol. 37, Iss. 4 — Feb. 15, 2012
  • pp: 707–709

Phase imaging flow cytometry using a focus-stack collecting microscope

Sai Siva Gorthi and Ethan Schonbrun  »View Author Affiliations


Optics Letters, Vol. 37, Issue 4, pp. 707-709 (2012)
http://dx.doi.org/10.1364/OL.37.000707


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Abstract

This letter introduces a fluidics-based focus-stack collecting microscope. A microfluidic device transports cells through the focal plane of a microscope, resulting in an efficient method to collect focus stacks of large collections of single cells. Images from the focus stacks are used to reconstruct the quantitative phase of cells with the transport-of-intensity-equation method. Using the phase imaging flow cytometer, we measure three-dimensional shape variations of red blood and leukemia cells.

© 2012 Optical Society of America

OCIS Codes
(100.5070) Image processing : Phase retrieval
(110.6880) Imaging systems : Three-dimensional image acquisition
(170.1530) Medical optics and biotechnology : Cell analysis

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: November 21, 2011
Revised Manuscript: January 9, 2012
Manuscript Accepted: January 9, 2012
Published: February 14, 2012

Virtual Issues
Vol. 7, Iss. 4 Virtual Journal for Biomedical Optics

Citation
Sai Siva Gorthi and Ethan Schonbrun, "Phase imaging flow cytometry using a focus-stack collecting microscope," Opt. Lett. 37, 707-709 (2012)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-37-4-707


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