OSA's Digital Library

Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 37, Iss. 4 — Feb. 15, 2012
  • pp: 752–754

Automatic three-dimensional tracking of particles with high-numerical-aperture digital lensless holographic microscopy

John F. Restrepo and Jorge Garcia-Sucerquia  »View Author Affiliations

Optics Letters, Vol. 37, Issue 4, pp. 752-754 (2012)

View Full Text Article

Enhanced HTML    Acrobat PDF (470 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We present an automatic procedure for 3D tracking of micrometer-sized particles with high-NA digital lensless holographic microscopy. The method uses a two-feature approach to search for the best focal planes and to distinguish particles from artifacts or other elements on the reconstructed stream of the holograms. A set of reconstructed images is axially projected onto a single image. From the projected image, the centers of mass of all the reconstructed elements are identified. Starting from the centers of mass, the morphology of the profile of the maximum intensity along the reconstruction direction allows for the distinguishing of particles from others elements. The method is tested with modeled holograms and applied to automatically track micrometer-sized bubbles in a sample of 4mm3 of soda.

© 2012 Optical Society of America

OCIS Codes
(100.2000) Image processing : Digital image processing
(100.6890) Image processing : Three-dimensional image processing
(090.1995) Holography : Digital holography
(100.4999) Image processing : Pattern recognition, target tracking

ToC Category:
Image Processing

Original Manuscript: November 28, 2011
Revised Manuscript: January 12, 2012
Manuscript Accepted: January 12, 2012
Published: February 15, 2012

Virtual Issues
Vol. 7, Iss. 4 Virtual Journal for Biomedical Optics

John F. Restrepo and Jorge Garcia-Sucerquia, "Automatic three-dimensional tracking of particles with high-numerical-aperture digital lensless holographic microscopy," Opt. Lett. 37, 752-754 (2012)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. T. G. Mason and D. A. Weitz, Phys. Rev. Lett. 74, 1250 (1995). [CrossRef]
  2. P. Tabeling, Introduction to Microfluidics (Oxford University, 2005).
  3. L. Tian, N. Loomis, J. A. Domínguez-Caballero, and G. Barbastathis, Appl. Opt. 49, 1549 (2010). [CrossRef]
  4. P. Langehanenberg, G. von Bally, and B. Kemper, 3D Res. 2, 1 (2011). [CrossRef]
  5. W. Xu, M. H. Jericho, H. J. Kreuzer, and I. A. Meinertzhagen, Opt. Lett. 28, 164 (2003). [CrossRef]
  6. J. Garcia-Sucerquia, W. Xu, P. Klages, S. M. Jericho, M. H. Jericho, and H. J. Kreuzer, Appl. Opt. 45, 836 (2006). [CrossRef]
  7. H. J. Kreuzer, K. Nakamura, A. Wierzbicki, H.-W. Fink, and H. Schmid, Ultramicroscopy 45, 381 (1992). [CrossRef]
  8. J. Garcia-Sucerquia, W. Xu, S. M. Jericho, M. H. Jericho, and H. J. Kreuzer, Optik 119, 419 (2008). [CrossRef]
  9. M. Heydt, A. Rosenhahn, M. Grunze, M. Pettitt, M. E. Callow, and J. A. Callow, J. Adhes. 83, 417 (2007). [CrossRef]
  10. N. Otsu, IEEE Trans. Syst. Man Cybern. 9, 62 (1979). [CrossRef]
  11. F. C. Cheong, B. Krishnatreya, and D. G. Grier, Opt. Express 18, 13563 (2010). [CrossRef]
  12. Y. Zhang, G. Shen, A. Schroder, and J. Kompenhans, Opt. Eng. 45, 075801 (2006). [CrossRef]
  13. W. Xu, M. H. Jericho, I. A. Meinertzhagen, and H. J. Kreuzer, Appl. Opt. 41, 5367 (2002). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1. Fig. 2. Fig. 3.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited