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Optics Letters

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  • Editor: Alan E. Willner
  • Vol. 37, Iss. 4 — Feb. 15, 2012
  • pp: 752–754

Automatic three-dimensional tracking of particles with high-numerical-aperture digital lensless holographic microscopy

John F. Restrepo and Jorge Garcia-Sucerquia  »View Author Affiliations


Optics Letters, Vol. 37, Issue 4, pp. 752-754 (2012)
http://dx.doi.org/10.1364/OL.37.000752


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Abstract

We present an automatic procedure for 3D tracking of micrometer-sized particles with high-NA digital lensless holographic microscopy. The method uses a two-feature approach to search for the best focal planes and to distinguish particles from artifacts or other elements on the reconstructed stream of the holograms. A set of reconstructed images is axially projected onto a single image. From the projected image, the centers of mass of all the reconstructed elements are identified. Starting from the centers of mass, the morphology of the profile of the maximum intensity along the reconstruction direction allows for the distinguishing of particles from others elements. The method is tested with modeled holograms and applied to automatically track micrometer-sized bubbles in a sample of 4mm3 of soda.

© 2012 Optical Society of America

OCIS Codes
(100.2000) Image processing : Digital image processing
(100.6890) Image processing : Three-dimensional image processing
(090.1995) Holography : Digital holography
(100.4999) Image processing : Pattern recognition, target tracking

ToC Category:
Image Processing

History
Original Manuscript: November 28, 2011
Revised Manuscript: January 12, 2012
Manuscript Accepted: January 12, 2012
Published: February 15, 2012

Virtual Issues
Vol. 7, Iss. 4 Virtual Journal for Biomedical Optics

Citation
John F. Restrepo and Jorge Garcia-Sucerquia, "Automatic three-dimensional tracking of particles with high-numerical-aperture digital lensless holographic microscopy," Opt. Lett. 37, 752-754 (2012)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-37-4-752


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