OSA's Digital Library

Optics Letters

Optics Letters

| RAPID, SHORT PUBLICATIONS ON THE LATEST IN OPTICAL DISCOVERIES

  • Editor: Alan E. Willner
  • Vol. 37, Iss. 5 — Mar. 1, 2012
  • pp: 812–814

Label-free 3D imaging of microstructure, blood, and lymphatic vessels within tissue beds in vivo

Zhongwei Zhi, Yeongri Jung, and Ruikang K. Wang  »View Author Affiliations


Optics Letters, Vol. 37, Issue 5, pp. 812-814 (2012)
http://dx.doi.org/10.1364/OL.37.000812


View Full Text Article

Enhanced HTML    Acrobat PDF (454 KB)





Browse Journals / Lookup Meetings

Browse by Journal and Year


   


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools

Share
Citations

Abstract

This Letter reports the use of an ultrahigh resolution optical microangiography (OMAG) system for simultaneous 3D imaging of microstructure and lymphatic and blood vessels without the use of an exogenous contrast agent. An automatic algorithm is developed to segment the lymphatic vessels from the microstructural images based on the fact that the lymph fluid is optically transparent. An OMAG system is developed that utilizes a broadband supercontinuum light source, providing an axial resolution of 2.3 μm and lateral resolution of 5.8 μm, capable of resolving the capillary vasculature and lymphatic vessels innervating microcirculatory tissue beds. Experimental demonstration is performed by showing detailed 3D lymphatic and blood vessel maps, coupled with morphology, within mouse ears in vivo.

© 2012 Optical Society of America

OCIS Codes
(170.3880) Medical optics and biotechnology : Medical and biological imaging
(170.4500) Medical optics and biotechnology : Optical coherence tomography

ToC Category:
Medical Optics and Biotechnology

History
Original Manuscript: December 8, 2011
Revised Manuscript: January 14, 2012
Manuscript Accepted: January 16, 2012
Published: February 21, 2012

Virtual Issues
Vol. 7, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Zhongwei Zhi, Yeongri Jung, and Ruikang K. Wang, "Label-free 3D imaging of microstructure, blood, and lymphatic vessels within tissue beds in vivo," Opt. Lett. 37, 812-814 (2012)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-37-5-812


Sort:  Author  |  Year  |  Journal  |  Reset  

References

  1. K. Alitalo, T. Tammela, and T. V. Petrova, Nature 438, 946 (2005). [CrossRef]
  2. M. S. Pepper, Clin. Canc. Res. 7, 462 (2001).
  3. F. Zhang, G. Niu, G. M. Lu, and X. Y. Chen, Mol. Imag. Biol. 13, 599 (2011).
  4. C. Kim, K. H. Song, F. Gao, and L. H. V. Wang, Radiology 255, 442 (2010). [CrossRef]
  5. D. Huang, E. A. Swanson, C. P. Lin, J. S. Schuman, W. G. Stinson, W. Chang, M. R. Hee, T. Flotte, K. Gregory, C. A. Puliafito, and J. G. Fujimoto, Science 254, 1178 (1991). [CrossRef]
  6. B. J. Vakoc, R. M. Lanning, J. A. Tyrrell, T. P. Padera, L. A. Bartlett, T. Stylianopoulos, L. L. Munn, G. J. Tearney, D. Fukumura, and B. E. Bouma, Nature Medicine 15, 1219 (2009). [CrossRef]
  7. Y. Jung, Z. Zhi, and R. K. Wang, J. Biomed. Opt. 15, 050501 (2010).
  8. Z. W. Zhi, J. Qin, L. An, and R. K. Wang, Opt. Lett. 36, 3169 (2011). [CrossRef]
  9. Z. Zhi, Y. Jung, Y. Jia, L. An, and R. K. Wang, Biomed. Opt. Express 2, 1059 (2011). [CrossRef]
  10. E. I. Galanzha, V. V. Tuchin, and V. P. Zharov, J. Biomed. Opt. 10, 054018 (2005). [CrossRef]
  11. M. J. Miller, S. H. Wei, I. Parker, and M. D. Cahalan, Science 296, 1869 (2002). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.

Figures

Fig. 1. Fig. 2. Fig. 3.
 

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited