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Optics Letters

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  • Editor: Alan E. Willner
  • Vol. 37, Iss. 5 — Mar. 1, 2012
  • pp: 827–829

Addressable discrete-line-scanning multiphoton microscopy based on a spatial light modulator

Wan Qin, Yonghong Shao, Honghai Liu, Xiang Peng, Hanben Niu, and Bruce Gao  »View Author Affiliations


Optics Letters, Vol. 37, Issue 5, pp. 827-829 (2012)
http://dx.doi.org/10.1364/OL.37.000827


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Abstract

We developed a novel addressable discrete-line-scanning multiphoton microscope with high lateral and axial resolutions based on a spatial light modulator. Our discrete-line-focus design eliminates the cross talk that occurs in conventional one-dimensional line-scanning multiphoton microscopies. Additionally, a phase-only spatial light modulator is able to scan only a sample’s target area by generating a specific discrete line focus according to the shape and location of the target area. Compared with other multiphoton microscopies, this technique shortens scanning time and minimizes photodamage by concentrating scanning energy and dwell time on the area of interest.

© 2012 Optical Society of America

OCIS Codes
(170.7160) Medical optics and biotechnology : Ultrafast technology
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:
Microscopy

History
Original Manuscript: October 3, 2011
Revised Manuscript: January 5, 2012
Manuscript Accepted: January 5, 2012
Published: February 22, 2012

Virtual Issues
Vol. 7, Iss. 5 Virtual Journal for Biomedical Optics

Citation
Wan Qin, Yonghong Shao, Honghai Liu, Xiang Peng, Hanben Niu, and Bruce Gao, "Addressable discrete-line-scanning multiphoton microscopy based on a spatial light modulator," Opt. Lett. 37, 827-829 (2012)
http://www.opticsinfobase.org/ol/abstract.cfm?URI=ol-37-5-827


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