OSA's Digital Library

Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 37, Iss. 5 — Mar. 1, 2012
  • pp: 827–829

Addressable discrete-line-scanning multiphoton microscopy based on a spatial light modulator

Wan Qin, Yonghong Shao, Honghai Liu, Xiang Peng, Hanben Niu, and Bruce Gao  »View Author Affiliations

Optics Letters, Vol. 37, Issue 5, pp. 827-829 (2012)

View Full Text Article

Enhanced HTML    Acrobat PDF (274 KB)

Browse Journals / Lookup Meetings

Browse by Journal and Year


Lookup Conference Papers

Close Browse Journals / Lookup Meetings

Article Tools



We developed a novel addressable discrete-line-scanning multiphoton microscope with high lateral and axial resolutions based on a spatial light modulator. Our discrete-line-focus design eliminates the cross talk that occurs in conventional one-dimensional line-scanning multiphoton microscopies. Additionally, a phase-only spatial light modulator is able to scan only a sample’s target area by generating a specific discrete line focus according to the shape and location of the target area. Compared with other multiphoton microscopies, this technique shortens scanning time and minimizes photodamage by concentrating scanning energy and dwell time on the area of interest.

© 2012 Optical Society of America

OCIS Codes
(170.7160) Medical optics and biotechnology : Ultrafast technology
(180.2520) Microscopy : Fluorescence microscopy
(180.6900) Microscopy : Three-dimensional microscopy
(180.4315) Microscopy : Nonlinear microscopy

ToC Category:

Original Manuscript: October 3, 2011
Revised Manuscript: January 5, 2012
Manuscript Accepted: January 5, 2012
Published: February 22, 2012

Virtual Issues
Vol. 7, Iss. 5 Virtual Journal for Biomedical Optics

Wan Qin, Yonghong Shao, Honghai Liu, Xiang Peng, Hanben Niu, and Bruce Gao, "Addressable discrete-line-scanning multiphoton microscopy based on a spatial light modulator," Opt. Lett. 37, 827-829 (2012)

Sort:  Author  |  Year  |  Journal  |  Reset  


  1. W. Denk, J. H. Strickler, and W. W. Webb, Science 248, 73 (1990). [CrossRef]
  2. G. J. Brakenhoff, J. Squier, T. Norris, A. C. Bliton, M. H. Wade, and B. Athey, J. Microsc. 181, 253 (1996). [CrossRef]
  3. A. Egner and S. W. Hell, J. Opt. Soc. Am. A 17, 1192 (2000). [CrossRef]
  4. S. W. Hell, V. Andresen, and A. Egner, Opt. Lett. 26, 75 (2001). [CrossRef]
  5. J. Bewersdorf, R. Pick, and S. W. Hell, Opt. Lett. 23, 655 (1998). [CrossRef]
  6. S. W. Hell and V. Andresen, J. Microsc. 202, 457 (2001). [CrossRef]
  7. L. Sacconi, E. Froner, R. Antolini, M. R. Taghizadeh, A. Choudhury, and F. S. Pavone, Opt. Lett. 28, 1918 (2003). [CrossRef]
  8. V. Nikolenko, B. O. Watson, R. Araya, A. Woodruff, D. S. Peterka, and R. Yuste, Front Neural Circuits 2, 5 (2008).
  9. R. W. Gerchber and W. O. Saxton, Optik 35, 237 (1972).
  10. M. Petran, M. Hadravsk, M. D. Egger, and R. Galambos, J. Opt. Soc. Am. 58, 661 (1968). [CrossRef]

Cited By

Alert me when this paper is cited

OSA is able to provide readers links to articles that cite this paper by participating in CrossRef's Cited-By Linking service. CrossRef includes content from more than 3000 publishers and societies. In addition to listing OSA journal articles that cite this paper, citing articles from other participating publishers will also be listed.


Fig. 1. Fig. 2. Fig. 3.

« Previous Article  |  Next Article »

OSA is a member of CrossRef.

CrossCheck Deposited