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Optics Letters


  • Editor: Alan E. Willner
  • Vol. 37, Iss. 6 — Mar. 15, 2012
  • pp: 1094–1096

Diffraction phase microscopy with white light

Basanta Bhaduri, Hoa Pham, Mustafa Mir, and Gabriel Popescu  »View Author Affiliations

Optics Letters, Vol. 37, Issue 6, pp. 1094-1096 (2012)

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We present white light diffraction phase microscopy (wDPM) as a quantitative phase imaging method that combines the single shot measurement benefit associated with off-axis methods, high temporal phase stability associated with common path geometries, and high spatial phase sensitivity due to the white light illumination. We propose a spatiotemporal filtering method that pushes the limit of the pathlength sensitivity to the subangstrom level at practical spatial and temporal bandwidths. We illustrate the utility of wDPM with measurements on red blood cell morphology and HeLa cell growth over 18 hours.

© 2012 Optical Society of America

OCIS Codes
(050.1950) Diffraction and gratings : Diffraction gratings
(070.6110) Fourier optics and signal processing : Spatial filtering
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(170.0180) Medical optics and biotechnology : Microscopy

ToC Category:
Medical Optics and Biotechnology

Original Manuscript: December 13, 2011
Revised Manuscript: January 24, 2012
Manuscript Accepted: January 24, 2012
Published: March 14, 2012

Virtual Issues
Vol. 7, Iss. 5 Virtual Journal for Biomedical Optics

Basanta Bhaduri, Hoa Pham, Mustafa Mir, and Gabriel Popescu, "Diffraction phase microscopy with white light," Opt. Lett. 37, 1094-1096 (2012)

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