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Optics Letters

Optics Letters


  • Editor: Alan E. Willner
  • Vol. 37, Iss. 7 — Apr. 1, 2012
  • pp: 1172–1174

Autofocusing based on wavelength dependence of diffraction in two-wavelength digital holographic microscopy

Peng Gao, Baoli Yao, Romano Rupp, Junwei Min, Rongli Guo, Baiheng Ma, Juanjuan Zheng, Ming Lei, Shaohui Yan, Dan Dan, and Tong Ye  »View Author Affiliations

Optics Letters, Vol. 37, Issue 7, pp. 1172-1174 (2012)

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An autofocusing method for two-wavelength digital holographic microscopy (TWDHM) based on the wavelength dependence of the diffraction process is proposed. Red and green lights are employed for the illumination of the TWDHM, and the generated holograms are recorded simultaneously by a color CCD camera. Due to the wavelength dependency of the diffraction process, the farther the reconstruction plane is from the image plane, the larger the difference is between the red and green light distributions. Thus, the image plane can be determined by finding the minimum of the variation between the red and green lights on their amplitude distributions. The feasibility of the proposed method is demonstrated by simulation and experiment.

© 2012 Optical Society of America

OCIS Codes
(090.2880) Holography : Holographic interferometry
(120.3180) Instrumentation, measurement, and metrology : Interferometry
(120.5050) Instrumentation, measurement, and metrology : Phase measurement
(180.3170) Microscopy : Interference microscopy

ToC Category:
Instrumentation, Measurement, and Metrology

Original Manuscript: September 30, 2011
Revised Manuscript: January 17, 2012
Manuscript Accepted: January 18, 2012
Published: March 23, 2012

Virtual Issues
Vol. 7, Iss. 6 Virtual Journal for Biomedical Optics

Peng Gao, Baoli Yao, Romano Rupp, Junwei Min, Rongli Guo, Baiheng Ma, Juanjuan Zheng, Ming Lei, Shaohui Yan, Dan Dan, and Tong Ye, "Autofocusing based on wavelength dependence of diffraction in two-wavelength digital holographic microscopy," Opt. Lett. 37, 1172-1174 (2012)

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