We report a miniaturized resonant/non-resonant multi-fiber raster scanner that is paired with a gradient-index lens assembly to achieve a compact and flexible multifocal multiphoton endoscope capable of longitudinal parallel image acquisition. Multiphoton images are obtained simultaneously at three axial depths, separated by $\ge 4.8\mathrm{\mu m}$, by incorporating three axially offset double clad optical fibers into the miniaturized scanner. The fabricated endoscope has an outer diameter of 3 mm, a rigid length of 4 cm, and acquires images at $4\text{frames}/\mathrm{s}$ per focal plane, with lateral and axial resolutions for two-photon imaging of 0.8 and 10 μm, respectively.

© 2012 Optical Society of America

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